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Literature summary extracted from

  • Loeffler, F.; Mueller, R.; Lingens, F.
    Purification and properties of 4-halobenzoate-coenzyme A ligase from Pseudomonas sp. CBS3 (1992), Biol. Chem. Hoppe-Seyler, 373, 1001-1007.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.33 Cu2+
-
 Pseudomonas sp.
6.2.1.33 Fe2+
-
 Pseudomonas sp.
6.2.1.33 SDS 1 mM, irreversibe Pseudomonas sp.
6.2.1.33 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.33 0.05 0.065 4-Chlorobenzoate
-
 Pseudomonas sp.
6.2.1.33 0.11 0.19 CoA
-
 Pseudomonas sp.
6.2.1.33 2.4 3.5 ATP
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.33 Co2+ 77% of the activation with Mg2+ Pseudomonas sp.
6.2.1.33 Fe2+ 70% of the activation with Mg2+ Pseudomonas sp.
6.2.1.33 Mg2+ required Pseudomonas sp.
6.2.1.33 Mg2+ maximal initial rate of catalysis is achieved with an Mg2+:ATP ratio of 1:0.66 to 1:1.33 Pseudomonas sp.
6.2.1.33 Mn2+ 87% of the activation with Mg2+ Pseudomonas sp.
6.2.1.33 Ni2+ 11% of the activation with Mg2+ Pseudomonas sp.
6.2.1.33 Zn2+ 23% of the activation with Mg2+ Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.33 57000
-
 2 * 57000, SDS-PAGE Pseudomonas sp.
6.2.1.33 86000 120000 gel filtration Pseudomonas sp.
6.2.1.33 125000
-
 native gradient PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.33 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.33
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.33 111.6
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
6.2.1.33 -20°C, stable for months Pseudomonas sp.
6.2.1.33 4°C, 0.1-2.5 mg of protein per ml, half-life: 21 days Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.33 4-Bromobenzoate + CoA + ATP at 105% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Bromobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ADP reaction with ADP at 7.5% of the reaction with ATP Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + phosphate
-
 ?
6.2.1.33 4-Chlorobenzoate + CoA + ATP negligible activity with UTP, CTP, and GTP Pseudomonas sp. 4-Chlorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Fluorobenzoate + CoA + ATP at 36% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Fluorobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Iodobenzoate + CoA + ATP at 90% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Iodobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Methoxybenzoate + CoA + ATP at 9% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Methoxybenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Methylbenzoate + CoA + ATP at 22% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Methylbenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 4-Nitrobenzoate + CoA + ATP at 7% of the activity with 4-chlorobenzoate Pseudomonas sp. 4-Nitrobenzoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.33 Benzoate + CoA + ATP at 5% of the activity with 4-chlorobenzoate Pseudomonas sp. Benzoyl-CoA + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.33 dimer 2 * 57000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.33 35
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.33 4
-
 0.1-2.5 mg of protein per ml, half-life: 21 days Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.33 8.4
-
-
 Pseudomonas sp.