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Literature summary extracted from

  • Lewis, J.A.; Jacobo, E.P.; Palmer, N.; Vermerris, W.; Sattler, S.E.; Brozik, J.A.; Sarath, G.; Kang, C.
    Structural and interactional analysis of the flavonoid pathway proteins chalcone synthase, chalcone isomerase and chalcone isomerase-like protein (2024), Int. J. Mol. Sci., 25, 5651.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expressed in Escherichia coli BL21(DE3) cells Panicum virgatum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.74 hanging drop vapor diffusion method, using 0.2 M sodium formate and 20% (v/v) PEG 3350 with 10 molar excesses of naringenin and CoA Panicum virgatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0001303
-
 4-coumaroyl-CoA pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 0.001121
-
 4-coumaroyl-CoA in the presence of chalcone isomerase and chalcone isomerase-like protein, pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 0.001321
-
 4-coumaroyl-CoA in the presence of chalcone isomerase-like protein, pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 0.001545
-
 4-coumaroyl-CoA in the presence of chalcone isomerase, pH and temperature not specified in the publication Panicum virgatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA Panicum virgatum
-
 4 CoA + naringenin chalcone + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Panicum virgatum A0A8T0PQ54 Blackwell switchgrass
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 Ni-NTA column chromatography and Superdex 200 gel filtration Panicum virgatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Panicum virgatum 4 CoA + naringenin chalcone + 3 CO2
-
 ?
2.3.1.74 additional information both chalcone isomerase and chalcone isomerase-lile protein physically interact with the enzyme in a non-competitive manner Panicum virgatum ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 CHS
-
 Panicum virgatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.74 0.00695
-
 4-coumaroyl-CoA pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 0.0505
-
 4-coumaroyl-CoA in the presence of chalcone isomerase-like protein, pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 0.175
-
 4-coumaroyl-CoA in the presence of chalcone isomerase, pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 3.04
-
 4-coumaroyl-CoA in the presence of chalcone isomerase and chalcone isomerase-like protein, pH and temperature not specified in the publication Panicum virgatum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.74 38.2
-
 4-coumaroyl-CoA in the presence of chalcone isomerase-like protein, pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 53.3
-
 4-coumaroyl-CoA pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 113.3
-
 4-coumaroyl-CoA in the presence of chalcone isomerase, pH and temperature not specified in the publication Panicum virgatum
2.3.1.74 2711
-
 4-coumaroyl-CoA in the presence of chalcone isomerase and chalcone isomerase-like protein, pH and temperature not specified in the publication Panicum virgatum