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Literature summary extracted from

  • Flor-Parra, I.; Sabido-Bozo, S.; Ikeda, A.; Hanaoka, K.; Aguilera-Romero, A.; Funato, K.; Muniz, M.; Lucena, R.
    The ceramide synthase subunit Lac1 regulates cell growth and size in fission yeast (2021), Int. J. Mol. Sci., 23, 303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.299 gene lac1, recombinant expression of C-terminally GFP-tagged enzyme, GFP or Tomato tagging at the C-terminus does not interfer with Lag1 localization in the cell, while GFP tagging interfers with Lac1 localization leading to a peculiar enzyme localization pattern Schizosaccharomyces pombe
2.3.1.299 gene lag1, recombinant expression of C-terminally GFP- or Tomato-tagged enzyme, GFP or Tomato tagging at the C-terminal does not interfer with Lag1 localization in the cell, while GFP tagging interfers with Lac1 localization leading to a peculiar enzyme localization pattern Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.299 additional information double deletion of lac1DELTA and lag1DELTA is inviable in normal conditions. Single mutant lac1DELTA, but not lag1DELTA, shows strong defects in cell proliferation at all temperatures in a spot assay. Growth defects of lac1DELTA are also observed when the cells are grown in liquid media. Growing cells at 25°C reveal a strong decrease in cell length at division in lac1DELTA cells. The decrease in cell size in lac1DELTA cells is partially reduced at 36°C. The Lag1 mutation has no significant effect on cell size at division in any condition tested Schizosaccharomyces pombe
2.3.1.299 additional information double deletion of lac1DELTA and lag1DELTA is inviable in normal conditions. Single mutant lac1DELTA, but not lag1DELTA, shows strong defects in cell proliferation at all temperatures in a spot assay. Growing cells at 25°C reveal a strong decrease in cell length at division in lac1DELTA cells. The decrease in cell size in lac1DELTA cells is partially reduced at 36°C. The Lag1 mutation has no significant effect on cell size at division in any condition tested Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 endoplasmic reticulum the ceramide synthase complex is localized at the endoplasmic reticulum Schizosaccharomyces pombe 5783
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2.3.1.299 endoplasmic reticulum fission yeast Lac1 and Lag1 co-localize at the endoplasmic reticulum, but their ER localizations are not interdependent Schizosaccharomyces pombe 5783
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2.3.1.299 endoplasmic reticulum fission yeast Lac1 and Lag1 co-localize at the endoplasmic reticulum, but their endoplasmic reticulum localizations are not interdependent Schizosaccharomyces pombe 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.299 additional information Schizosaccharomyces pombe fission-yeast-specific ceramides are dihydroceramides (DH-Cer). Schizosaccahromyces pombe lipid A (DH-Cer1) is a short-chain DH-Cer, while lipids B (DH-Cer2) and C (DH-Cer3) are long-chain DH-Cer ?
-
-
2.3.1.299 additional information Schizosaccharomyces pombe 972 fission-yeast-specific ceramides are dihydroceramides (DH-Cer). Schizosaccahromyces pombe lipid A (DH-Cer1) is a short-chain DH-Cer, while lipids B (DH-Cer2) and C (DH-Cer3) are long-chain DH-Cer ?
-
-
2.3.1.299 additional information Schizosaccharomyces pombe ATCC 24843 fission-yeast-specific ceramides are dihydroceramides (DH-Cer). Schizosaccahromyces pombe lipid A (DH-Cer1) is a short-chain DH-Cer, while lipids B (DH-Cer2) and C (DH-Cer3) are long-chain DH-Cer ?
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-
2.3.1.299 stearoyl-CoA + dihydrosphinganine Schizosaccharomyces pombe
-
 N-(stearoyl)-dihydroceramide + CoA
-
 ?
2.3.1.299 stearoyl-CoA + dihydrosphinganine Schizosaccharomyces pombe 972
-
 N-(stearoyl)-dihydroceramide + CoA
-
 ?
2.3.1.299 stearoyl-CoA + dihydrosphinganine Schizosaccharomyces pombe ATCC 24843
-
 N-(stearoyl)-dihydroceramide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Schizosaccharomyces pombe O59735 AND P78970 O59735: ceramide synthase subunit Lac1, P78970: ceramide synthase subunit Lag1
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2.3.1.24 Schizosaccharomyces pombe ATCC 24843 O59735 AND P78970 O59735: ceramide synthase subunit Lac1, P78970: ceramide synthase subunit Lag1
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2.3.1.299 Schizosaccharomyces pombe O59735 cf. EC 2.3.1.24
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2.3.1.299 Schizosaccharomyces pombe P78970 cf. EC 2.3.1.24
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2.3.1.299 Schizosaccharomyces pombe 972 O59735 cf. EC 2.3.1.24
-
2.3.1.299 Schizosaccharomyces pombe 972 P78970 cf. EC 2.3.1.24
-
2.3.1.299 Schizosaccharomyces pombe ATCC 24843 O59735 cf. EC 2.3.1.24
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2.3.1.299 Schizosaccharomyces pombe ATCC 24843 P78970 cf. EC 2.3.1.24
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.299 additional information fission-yeast-specific ceramides are dihydroceramides (DH-Cer). Schizosaccahromyces pombe lipid A (DH-Cer1) is a short-chain DH-Cer, while lipids B (DH-Cer2) and C (DH-Cer3) are long-chain DH-Cer Schizosaccharomyces pombe ?
-
-
2.3.1.299 additional information fission-yeast-specific ceramides are dihydroceramides (DH-Cer). Schizosaccahromyces pombe lipid A (DH-Cer1) is a short-chain DH-Cer, while lipids B (DH-Cer2) and C (DH-Cer3) are long-chain DH-Cer Schizosaccharomyces pombe 972 ?
-
-
2.3.1.299 additional information fission-yeast-specific ceramides are dihydroceramides (DH-Cer). Schizosaccahromyces pombe lipid A (DH-Cer1) is a short-chain DH-Cer, while lipids B (DH-Cer2) and C (DH-Cer3) are long-chain DH-Cer Schizosaccharomyces pombe ATCC 24843 ?
-
-
2.3.1.299 stearoyl-CoA + dihydrosphinganine
-
 Schizosaccharomyces pombe N-(stearoyl)-dihydroceramide + CoA
-
 ?
2.3.1.299 stearoyl-CoA + dihydrosphinganine
-
 Schizosaccharomyces pombe 972 N-(stearoyl)-dihydroceramide + CoA
-
 ?
2.3.1.299 stearoyl-CoA + dihydrosphinganine
-
 Schizosaccharomyces pombe ATCC 24843 N-(stearoyl)-dihydroceramide + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase
-
 Schizosaccharomyces pombe
2.3.1.24 LAC1 ceramide synthase subunit Schizosaccharomyces pombe
2.3.1.24 LAG1 ceramide synthase subunit Schizosaccharomyces pombe
2.3.1.299 LAC1
-
 Schizosaccharomyces pombe
2.3.1.299 LAG1
-
 Schizosaccharomyces pombe
2.3.1.299 sphingosine N-acyltransferase LAC1 SwissProt Schizosaccharomyces pombe
2.3.1.299 sphingosine N-acyltransferase LAG1 SwissProt Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
2.3.1.24 physiological function the ceramide synthase subunit Lac1 regulates cell growth and size in Schizosaccharomyces pombe Schizosaccharomyces pombe
2.3.1.299 metabolism ceramide synthase is composed of three different subunits, namely Lac1, Lag1, and the regulatory subunit Lip1. Both Lac1 and Lag1 catalyze N-acylation of dihydrosphingosine (DHS) or phytosphingosine (PHS) to a C26 fatty acid, producing dihydroceramide (DHCer, or Cer-A) or phytoceramide (PHCer or Cer-B). Specific ceramide and sphingolipid species produced by Lac1 are required for normal control of cell growth and size in fission yeast. It is possible that fission yeast Lac1 carries major weight in ceramide and complex sphingolipid canonical production, while Lag1 is only necessary for specific situations Schizosaccharomyces pombe
2.3.1.299 additional information Lac1, Lag1, and Lip1 are subunits of ceramide synthase 1 in yeast. Lac1 and Lag1 interact in vivo Schizosaccharomyces pombe
2.3.1.299 physiological function all cells require mechanisms to measure growth and trigger cell division when sufficient growth has occurred. Fission yeast grows in length by linear extension during G2 and divides at a constant cell size. Ceramide synthase subunit Lac1 regulates cell growth and size in fission yeast. Both Lac1 and Lag1 catalyze N-acylation of dihydrosphingosine (DHS) or phytosphingosine (PHS) to a C26 fatty acid, producing dihydroceramide (DHCer, or Cer-A) or phytoceramide (PHCer or Cer-B). Specific ceramide and sphingolipid species produced by Lac1 are required for normal control of cell growth and size in fission yeast, while Lag1 is not. Enzymes Lac1 and Lag1 generate different species of ceramides and complex sphingolipids Schizosaccharomyces pombe