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Literature summary extracted from

  • Saini, M.; Kashyap, A.; Bindal, S.; Saini, K.; Gupta, R.
    Bacterial gamma-glutamyl transpeptidase, an emerging biocatalyst insights into structure-function relationship and its biotechnological applications (2021), Front. Microbiol., 12, 641251.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.2.2 food industry some essential amino acids like Phe, Leu, Val and His, taste bitter when consumed orally. The addition of gamma-glutamyl moiety to these amino acids using Escherichia coli K-12 gamma-glutamyltransferase has been reported to dramatically reduce their bitterness and improve their taste preference due to the introduction of a refreshing sour flavor, making them suitable for oral consumption as amino acid supplements Escherichia coli
2.3.2.2 synthesis enzymatic synthesis of L-theanine Pseudomonas nitroreducens
2.3.2.2 synthesis the enzyme is employed in enzymatic synthesis of L-theanine, gamma-glutamyl-taurine or gamma-D-glutamyl-L-tryptophan (an immunomodulatory dipeptide with broadspectrum stimulatory activities) Escherichia coli
2.3.2.2 synthesis enzymatic synthesis of L-theanine Bacillus subtilis
2.3.2.2 synthesis enzymatic synthesis of L-theanine Bacillus licheniformis
2.3.2.2 synthesis enzymatic synthesis of L-theanine Bacillus amyloliquefaciens
2.3.2.2 synthesis enzymatic synthesis of L-theanine Bacillus pumilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expression in Bacillus subtilis 168 Bacillus pumilus
2.3.2.2 expression in Bacillus subtilis KCTC 3135 Bacillus amyloliquefaciens
2.3.2.2 expression in Escherichia coli Bacillus licheniformis
2.3.2.2 expression in Escherichia coli BL21 (DE3) Deinococcus radiodurans
2.3.2.2 expression in Escherichia coli rosetta Picrophilus oshimae
2.3.2.2 expression in Escherichia coli Rosetta-gami B (DE3) Pseudomonas nitroreducens
2.3.2.2 expression in gamma-glutamyltransferase deficient Escherichia coli K-12 Escherichia coli
2.3.2.2 expression in sporulation-deficient Bacillus subtilis 168 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 extracellular
-
 Bacillus licheniformis
-
-
2.3.2.2 periplasm
-
 Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus amyloliquefaciens
-
-
-
2.3.2.2 Bacillus licheniformis
-
-
-
2.3.2.2 Bacillus pumilus
-
-
-
2.3.2.2 Bacillus subtilis
-
-
-
2.3.2.2 Deinococcus radiodurans
-
-
-
2.3.2.2 Escherichia coli P18956
-
-
2.3.2.2 Escherichia coli K12 P18956
-
-
2.3.2.2 Picrophilus oshimae
-
-
-
2.3.2.2 Picrophilus oshimae DSM 9790
-
-
-
2.3.2.2 Pseudomonas nitroreducens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Bacillus pumilus
2.3.2.2
-
 Bacillus subtilis
2.3.2.2 one-step purfication, Ni-NTA affinity chromatography utilizing C-terminal His6 tag Deinococcus radiodurans
2.3.2.2 one-step purfication, Ni-NTA affinity chromatography utilizing C-terminal His6 tag Picrophilus oshimae
2.3.2.2 one-step purification, Ni-NTA affinity chromatography utilizing N-terminal His6 tag Bacillus licheniformis
2.3.2.2 two-step purification, ammonium sulfate precipitation and chromatofocusing Escherichia coli
2.3.2.2 two-step purification, DEAE cellulofine and Butyl FF column chromatography Pseudomonas nitroreducens

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 gamma-glutamyl transpeptidase
-
 Pseudomonas nitroreducens
2.3.2.2 gamma-glutamyl transpeptidase
-
 Escherichia coli
2.3.2.2 gamma-glutamyl transpeptidase
-
 Bacillus subtilis
2.3.2.2 gamma-glutamyl transpeptidase
-
 Bacillus licheniformis
2.3.2.2 gamma-glutamyl transpeptidase
-
 Bacillus amyloliquefaciens
2.3.2.2 gamma-glutamyl transpeptidase
-
 Bacillus pumilus
2.3.2.2 gamma-glutamyl transpeptidase
-
 Deinococcus radiodurans
2.3.2.2 gamma-glutamyl transpeptidase
-
 Picrophilus oshimae
2.3.2.2 GGT
-
 Pseudomonas nitroreducens
2.3.2.2 GGT
-
 Escherichia coli
2.3.2.2 GGT
-
 Bacillus subtilis
2.3.2.2 GGT
-
 Bacillus licheniformis
2.3.2.2 GGT
-
 Bacillus amyloliquefaciens
2.3.2.2 GGT
-
 Bacillus pumilus
2.3.2.2 GGT
-
 Deinococcus radiodurans
2.3.2.2 GGT
-
 Picrophilus oshimae

General Information

EC Number General Information Comment Organism
2.3.2.2 physiological function putative physiological role in glutathione utilization as a source of amino acid and nitrogen under nutrient limiting conditions Escherichia coli