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Literature summary extracted from

  • Lu, X.F.; Diao, H.J.; Wu, Z.M.; Zhang, Z.L.; Zheng, R.C.; Zheng, Y.G.
    Engineering of reaction specificity, enantioselectivity, and catalytic activity of nitrilase for highly efficient synthesis of pregabalin precursor (2022), Biotechnol. Bioeng., 119, 2399-2412.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 M127I/C237S mutation leads to nearly 3.9fold improvement in catalytic activity Brassica rapa
3.5.5.1 M127I/C237S mutation leads to nearly 3.9fold improvement in catalytic activity Arabis alpina
3.5.5.1 V82A/M127I/C237S 29.1% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82A/M127I/C237S 29.1% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82C/M127I/C237S 122.2% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82C/M127I/C237S 122.2% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82F/M127I/C237S 74.2% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82F/M127I/C237S 74.2% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82I/M127I/C237S 82.9% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82I/M127I/C237S 82.9% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82L/M127I 86.5% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82L/M127I 86.5% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82L/M127I/C237S the variant displays higher enantioselectivity, increased enzyme activity (5.4fold) and reduced amide formation (from 15.8% to 1.9%) compared with BaNIT Brassica rapa
3.5.5.1 V82L/M127I/C237S 139.7% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82L/M127I/C237S the variant displays higher enantioselectivity, increased enzyme activity (5.4fold) and reduced amide formation (from 15.8% to 1.9%) compared with BaNIT Arabis alpina
3.5.5.1 V82L/M127I/C237S 139.7% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82M/M127I/C237S 174.6% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82M/M127I/C237S 174.6% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina
3.5.5.1 V82Y/M127I/C237S 8.2% relative activity as compared to mutant enzyme M127I/C237S Brassica rapa
3.5.5.1 V82Y/M127I/C237S 8.2% relative activity as compared to mutant enzyme M127I/C237S Arabis alpina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 3.1
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant V82L/M127I/C237S Brassica rapa
3.5.5.1 3.1
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant V82L/M127I/C237S Arabis alpina
3.5.5.1 7.76
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant M127I/C237S Brassica rapa
3.5.5.1 7.76
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant M127I/C237S Arabis alpina
3.5.5.1 10.7
-
 isobutylsuccinonitrile pH 8.0, 30°C, chimeric enzyme BaNIT Brassica rapa
3.5.5.1 10.7
-
 isobutylsuccinonitrile pH 8.0, 30°C, chimeric enzyme BaNIT Arabis alpina

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Arabis alpina
-
-
-
3.5.5.1 Brassica rapa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
 Arabis alpina
3.5.5.1
-
 Brassica rapa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 isobutylsuccinonitrile + 2 H2O the by-product (S)-3-cyano-5-methylhexanoic amide is also formed Brassica rapa isobutylsuccinic acid + NH3
-
 ?
3.5.5.1 isobutylsuccinonitrile + 2 H2O the by-product (S)-3-cyano-5-methylhexanoic amide is also formed Arabis alpina isobutylsuccinic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 AaNIT
-
 Arabis alpina
3.5.5.1 BaNIT chimera using nitrilase from Arabis alpine (AaNIT) and Brassica rapa () Brassica rapa
3.5.5.1 BaNIT chimera using nitrilase from Arabis alpine (AaNIT) and Brassica rapa (BrNIT) Arabis alpina
3.5.5.1 BrNIT
-
 Brassica rapa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.1 10.3
-
 isobutylsuccinonitrile pH 8.0, 30°C, chimeric enzyme BaNIT Brassica rapa
3.5.5.1 10.3
-
 isobutylsuccinonitrile pH 8.0, 30°C, chimeric enzyme BaNIT Arabis alpina
3.5.5.1 24.3
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant V82L/M127I/C237S Brassica rapa
3.5.5.1 24.3
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant V82L/M127I/C237S Arabis alpina
3.5.5.1 37.7
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant M127I/C237S Brassica rapa
3.5.5.1 37.7
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant M127I/C237S Arabis alpina

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.5.1 0.96
-
 isobutylsuccinonitrile pH 8.0, 30°C, chimeric enzyme BaNIT Brassica rapa
3.5.5.1 0.96
-
 isobutylsuccinonitrile pH 8.0, 30°C, chimeric enzyme BaNIT Arabis alpina
3.5.5.1 4.9
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant M127I/C237S Brassica rapa
3.5.5.1 4.9
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant M127I/C237S Arabis alpina
3.5.5.1 7.8
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant V82L/M127I/C237S Brassica rapa
3.5.5.1 7.8
-
 isobutylsuccinonitrile pH 8.0, 30°C, BaNIT variant V82L/M127I/C237S Arabis alpina