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Literature summary extracted from

  • Zhang, Q.; Lu, X.F.; Zhang, Y.; Tang, X.L.; Zheng, R.C.; Zheng, Y.G.
    Development of a robust nitrilase by fragment swapping and semi-rational design for efficient biosynthesis of pregabalin precursor (2020), Biotechnol. Bioeng., 117, 318-329.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.5.1 L223Q/H263D/Q279E development of a robust nitrilase by fragment swapping and semi-rational design for efficient biosynthesis of pregabalin precursor. A chimera (BaNIT) harboring 12 amino acid substitutions is obtained using nitrilase from Arabis alpine (AaNIT) and Brassica rapa (BrNIT) as parent enzymes, which exhibits higher enantioselectivity and activity toward isobutylsuccinonitrile for the biosynthesis of pregabalin precursor. Semi-rational design is executed on BaNIT to further generate variant BaNIT/L223Q/H263D/Q279E with the concurrent improvement of activity, enantioselectivity, and solubility. The robust nitrilase displays a 5.4fold, 3.3fold, and 2.8fold increase in whole-cell activity, catalytic efficiency, and solubility, respectively Arabis alpina
3.5.5.1 L223Q/H263D/Q279E development of a robust nitrilase by fragment swapping and semi-rational design for efficient biosynthesis of pregabalin precursor. A chimera (BaNIT) harboring 12 amino acid substitutions is obtained using nitrilase from Arabis alpine (AaNIT) and Brassica rapa (BrNIT) as parent enzymes, which exhibits higher enantioselectivity and activity toward isobutylsuccinonitrile for the biosynthesis of pregabalin precursor. Semi-rational design is executed on BaNIT to further generate variant BaNIT/L223Q/H263D/Q279E with the concurrent improvement of activity, enantioselectivity, and solubility. The robust nitrilase displays a 5.4fold, 3.3fold, and 2.8fold increase in whole-cell activity, catalytic efficiency, and solubility, respectively Brassica rapa

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Arabis alpina
-
-
-
3.5.5.1 Brassica rapa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 (+/-)-isobutylsuccinonitrile + 2 H2O
-
 Arabis alpina (S)-3-cyano-5-methyl hexanoic acid
-
 ?
3.5.5.1 (+/-)-isobutylsuccinonitrile + H2O
-
 Brassica rapa (S)-3-cyano-5-methyl hexanoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 AaNIT
-
 Arabis alpina
3.5.5.1 BaNIT chimera harboring 12 amino acid substitutions is obtained using nitrilase from Arabis alpine (AaNIT) and Brassica rapa (BrNIT) Arabis alpina
3.5.5.1 BaNIT chimera harboring 12 amino acid substitutions is obtained using nitrilase from Arabis alpine (AaNIT) and Brassica rapa (BrNIT) Brassica rapa
3.5.5.1 BrNIT
-
 Brassica rapa