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Literature summary extracted from

  • Shcherbakova, T.A.; Baldin, S.M.; Shumkov, M.S.; Gushchina, I.V.; Nilov, D.K.; Svedas, V.K.
    Isolation and biochemical characterization of recombinant transketolase from Mycobacterium tuberculosis (2022), Acta naturae, 14, 93-97.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 expression of His-tagged protein in Escherichia coli. Addition of thiamine diphosphate during expression increases the holoenzyme content from 50% to 75% Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.03
-
 D-xylulose 5-phosphate pH 7.6, 25°C Mycobacterium tuberculosis
2.2.1.1 0.134
-
 D-ribose 5-phosphate pH 7.6, 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Ca2+ rates of apoenzyme activation and holoenzyme formation are higher in the presence of Ca2+ ions than in the presence of Mg2+ ions Mycobacterium tuberculosis
2.2.1.1 Mg2+ rates of apoenzyme activation and holoenzyme formation are higher in the presence of Ca2+ ions than in the presence of Mg2+ ions Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Mycobacterium tuberculosis P9WG25
-
-
2.2.1.1 Mycobacterium tuberculosis H37Rv P9WG25
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 about 50% of the resulting protein expressed in Escherichia coli is the apoenzyme, which rapidly loses its activity during isolation and purification. Addition of thiamine diphosphate during expression increases the holoenzyme content to 75%. Enzyme can be purified from the cofactor by precipitation with ammonium sulfate at pH 3.5 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 Mycobacterium tuberculosis sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 Mycobacterium tuberculosis H37Rv sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.1 Rv1449c
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate Km value in presence of Mg2+ is 0.057 mM, in presence of Ca2+ 0.003 mM Mycobacterium tuberculosis