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Literature summary extracted from

  • Tirkkonen, H.; Brown, K.V.; Niemczura, M.; Faudemer, Z.; Brown, C.; Ponomareva, L.V.; Helmy, Y.A.; Thorson, J.S.; Nybo, S.E.; Metsae-Ketelae, M.; Shaaban, K.A.
    Engineering BioBricks for deoxysugar biosynthesis and generation of new tetracenomycins (2023), ACS omega, 8, 21237-21253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.331 the BioBricks cloning system in Streptomyces coelicolor M1146::cos16F4iE is an excellent approach for the prototyping of sugar nucleotide pathways in vivo and is a launching pad for deeper understanding of structure-activity relationships. The BioBricks expression platform is used to engineer biosynthesis for D-configured TDP-deoxysugars, including 8-O-D-glucosyl-tetracenomycin C, 8-O-D-olivosyl-tetracenomycin C, 8-O-D-mycarosyl-tetracenomycin C, and 8-O-D-digitoxosyl-tetracenomycin Streptomyces olivaceus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.331 dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C Streptomyces olivaceus
-
 dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
-
 ?
2.4.1.331 dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C Streptomyces olivaceus Tü2353
-
 dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.331 Streptomyces olivaceus Q9F2F9
-
-
2.4.1.331 Streptomyces olivaceus Tü2353 Q9F2F9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.331 dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C
-
 Streptomyces olivaceus dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
-
 ?
2.4.1.331 dTDP-beta-L-rhamnose + 8-demethyltetracenomycin C
-
 Streptomyces olivaceus Tü2353 dTDP + 8-demethyl-8-alpha-L-rhamnosyltetracenomycin C
-
 ?