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Literature summary extracted from

  • Mickolajczyk, K.J.; Olinares, P.D.B.; Niu, Y.; Chen, N.; Warrington, S.E.; Sasaki, Y.; Walz, T.; Chait, B.T.; Kapoor, T.M.
    Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1 (2020), Proc. Natl. Acad. Sci. USA, 117, 18459-18469.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.2 3-((2-[3-(3-butyn-1-yl)-3H-diazirin-3-yl]ethyl)sulfanyl)-7-methyl-2H-[1,2,4]triazino[5,6-b]indole i.e. Rbin-XL Schizosaccharomyces pombe
5.6.1.2 3-[(2-methyl-2-propen-1-yl)sulfanyl]-2H-[1,2,4]triazino[5,6-b]indole i.e. Rbin-1, non-competitive inhibition Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.2 0.00025
-
 ATP full-length enzyme, at pH 7.5 and 37°C Schizosaccharomyces pombe
5.6.1.2 0.00053
-
 ATP C-terminally truncated enzyme, at pH 7.5 and 37°C Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.2 Mg2+ required Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.2 540000
-
 full-length enzyme, gel filtration Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Schizosaccharomyces pombe O94248
-
-
5.6.1.2 Schizosaccharomyces pombe 972 O94248
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.2 HisTrap column chromatography, HiTrap Q or MonoQ column chromatography, and Superose 6 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.2 ATP + ribosome 60S subunit
-
 Schizosaccharomyces pombe ?
-
 ?
5.6.1.2 ATP + ribosome 60S subunit
-
 Schizosaccharomyces pombe 972 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.2 dynein-like AAA motor
-
 Schizosaccharomyces pombe
5.6.1.2 Mdn1
-
 Schizosaccharomyces pombe
5.6.1.2 midasin
-
 Schizosaccharomyces pombe
5.6.1.2 Rea1
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6.1.2 2.4
-
 ATP full-length enzyme, at pH 7.5 and 37°C Schizosaccharomyces pombe
5.6.1.2 2.6
-
 ATP C-terminally truncated enzyme, at pH 7.5 and 37°C Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.1.2 ATP
-
 Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
5.6.1.2 physiological function the enzyme uses the energy from ATP hydrolysis to physically reshape and remodel, and thus mature, the 60S subunit of the ribosome Schizosaccharomyces pombe