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Literature summary extracted from

  • Freeman, M.; Moshos, K.; Bodner, M.; Li, R.; Townsend, C.
    Four enzymes define the incorporation of coenzyme A in thienamycin biosynthesis (2008), Proc. Natl. Acad. Sci. USA, 105, 11128-11133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.110 expressed in Escherichia coli BL21(DE3) pLysS cells Streptantibioticus cattleyicolor
3.6.1.77 expressed in Escherichia coli Rosetta2 (DE3) cells Streptantibioticus cattleyicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.77 Mg2+ 10 mM used in assay conditions Streptantibioticus cattleyicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.110 4'-phosphopantetheine + H2O Streptantibioticus cattleyicolor
-
 pantetheine + phosphate
-
 ?
3.6.1.77 coenzyme A + H2O Streptantibioticus cattleyicolor
-
 adenosine 3',5'-bisphosphate + 4'-phosphopantetheine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.110 Streptantibioticus cattleyicolor Q83XP6
-
-
3.6.1.77 Streptantibioticus cattleyicolor Q83XN6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.110 Ni-NTA column chromatography Streptantibioticus cattleyicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.110 4'-phosphopantetheine + H2O
-
 Streptantibioticus cattleyicolor pantetheine + phosphate
-
 ?
3.1.3.110 additional information the enzyme has a markedly reduced cleavage rate for prederivatized 4-phosphopantetheine Streptantibioticus cattleyicolor ?
-
-
3.6.1.77 coenzyme A + H2O
-
 Streptantibioticus cattleyicolor adenosine 3',5'-bisphosphate + 4'-phosphopantetheine
-
 ?
3.6.1.77 additional information CoA prederivatized with 2-chloro-1-methylquinolinium tetrafluoroborate is not a substrate for the enzyme Streptantibioticus cattleyicolor ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.1.3.110 thnH
-
 Streptantibioticus cattleyicolor
3.6.1.77 THNR
-
 Streptantibioticus cattleyicolor