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Literature summary extracted from

  • Martinez-Tobin, D.; Waters, B.; Elias, A.; Sauvageau, D.
    Streamlined production, purification, and characterization of recombinant extracellular polyhydroxybutyrate depolymerases (2020), MicrobiologyOpen, 9, e1001.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 gene phaZ, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B(DE3) from a vector including an N-terminal peIB leader sequence for periplasm localization of the protein Comamonas testosteroni
3.1.1.75 gene phaZ, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B(DE3) from a vector including an N-terminal peIB leader sequence for periplasm localization of the protein Cupriavidus sp. T1
3.1.1.75 gene phaZ, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B(DE3) from a vector including an N-terminal peIB leader sequence for periplasm localization of the protein Marinobacter algicola
3.1.1.75 gene phaZ, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B(DE3) from a vector including an N-terminal peIB leader sequence for periplasm localization of the protein Ralstonia pickettii
3.1.1.75 gene phaZ, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta-gami B(DE3) from a vector including an N-terminal peIB leader sequence for periplasm localization of the protein Stutzerimonas stutzeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Comamonas testosteroni
-
-
3.1.1.75 extracellular
-
 Cupriavidus sp. T1
-
-
3.1.1.75 extracellular
-
 Marinobacter algicola
-
-
3.1.1.75 extracellular
-
 Stutzerimonas stutzeri
-
-
3.1.1.75 extracellular
-
 Ralstonia pickettii
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Comamonas testosteroni
-
 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Cupriavidus sp. T1
-
 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Marinobacter algicola
-
 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Stutzerimonas stutzeri
-
 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Ralstonia pickettii
-
 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Comamonas testosteroni 31A
-
 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O Marinobacter algicola DG893
-
 (R)-3-hydroxybutyric acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Comamonas testosteroni Q5Q138 isolated from soil from a greenhouse
-
3.1.1.75 Comamonas testosteroni 31A Q5Q138 isolated from soil from a greenhouse
-
3.1.1.75 Cupriavidus sp. T1
-
 isolated from activated sludge obtained from the Toba sewage-treatment plant, Kyoto, Japan
-
3.1.1.75 Marinobacter algicola A6EXA3 isolated from laboratory culture of dinoflagellate Gymnodinium catenatum YC499B15
-
3.1.1.75 Marinobacter algicola DG893 A6EXA3 isolated from laboratory culture of dinoflagellate Gymnodinium catenatum YC499B15
-
3.1.1.75 Ralstonia pickettii Q52155 isolated from atmosphere in the laboratory, Japan
-
3.1.1.75 Stutzerimonas stutzeri O82950 Pseudomonas stutzeri, isolated from seawater, Jogashima, Kanagawa Pref., Japan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 recombinant extracellular C-terminally His-tagged enzyme from Escherichia coli strain Rosetta-gami B(DE3) by nickel affinity chromatography and ultrafiltration Comamonas testosteroni
3.1.1.75 recombinant extracellular C-terminally His-tagged enzyme from Escherichia coli strain Rosetta-gami B(DE3) by nickel affinity chromatography and ultrafiltration Cupriavidus sp. T1
3.1.1.75 recombinant extracellular C-terminally His-tagged enzyme from Escherichia coli strain Rosetta-gami B(DE3) by nickel affinity chromatography and ultrafiltration Ralstonia pickettii
3.1.1.75 recombinant extracellular C-terminally His-tagged enzyme from Escherichia coli strain Rosetta-gami B(DE3) by nickel affinity chromatography and ultrafiltration Stutzerimonas stutzeri
3.1.1.75 recombinant extracellular C-terminally His-tagged enzyme from Escherichia coli strain Rosetta-gami B(DE3) by nickel affinity chromatography, gel filtration and ultrafiltration Marinobacter algicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Comamonas testosteroni ?
-
-
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Cupriavidus sp. T1 ?
-
-
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Marinobacter algicola ?
-
-
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Stutzerimonas stutzeri ?
-
-
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Ralstonia pickettii ?
-
-
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Comamonas testosteroni 31A ?
-
-
3.1.1.75 additional information degradation activity of different rPhaZs is compared using a simple PHB plate-based method, adapted to test for various pH and temperatures Marinobacter algicola DG893 ?
-
-
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Comamonas testosteroni (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Cupriavidus sp. T1 (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Marinobacter algicola (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Stutzerimonas stutzeri (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Ralstonia pickettii (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Comamonas testosteroni 31A (R)-3-hydroxybutyric acid
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutyric acid] + H2O
-
 Marinobacter algicola DG893 (R)-3-hydroxybutyric acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 extracellular polyhydroxybutyrate depolymerase
-
 Comamonas testosteroni
3.1.1.75 extracellular polyhydroxybutyrate depolymerase
-
 Cupriavidus sp. T1
3.1.1.75 extracellular polyhydroxybutyrate depolymerase
-
 Marinobacter algicola
3.1.1.75 extracellular polyhydroxybutyrate depolymerase
-
 Stutzerimonas stutzeri
3.1.1.75 extracellular polyhydroxybutyrate depolymerase
-
 Ralstonia pickettii
3.1.1.75 PhaZ
-
 Comamonas testosteroni
3.1.1.75 PhaZ
-
 Cupriavidus sp. T1
3.1.1.75 PhaZ
-
 Marinobacter algicola
3.1.1.75 PhaZ
-
 Stutzerimonas stutzeri
3.1.1.75 PhaZ
-
 Ralstonia pickettii
3.1.1.75 phaZCsp
-
 Cupriavidus sp. T1
3.1.1.75 phaZCte
-
 Comamonas testosteroni
3.1.1.75 phaZMal
-
 Marinobacter algicola
3.1.1.75 phaZPst
-
 Stutzerimonas stutzeri
3.1.1.75 phaZRsp
-
 Ralstonia pickettii
3.1.1.75 PHB depolymerase
-
 Comamonas testosteroni
3.1.1.75 PHB depolymerase
-
 Cupriavidus sp. T1
3.1.1.75 PHB depolymerase
-
 Marinobacter algicola
3.1.1.75 PHB depolymerase
-
 Stutzerimonas stutzeri
3.1.1.75 PHB depolymerase
-
 Ralstonia pickettii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 37
-
 assay at Comamonas testosteroni
3.1.1.75 37
-
 assay at Cupriavidus sp. T1
3.1.1.75 37
-
 assay at Marinobacter algicola
3.1.1.75 37
-
 assay at Ralstonia pickettii
3.1.1.75 37
-
 assay at Stutzerimonas stutzeri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.75 15 37 inactive at 15°C, active at 37°C Comamonas testosteroni
3.1.1.75 15 37 inactive at 15°C, active at 37°C Cupriavidus sp. T1
3.1.1.75 15 37 inactive at 15°C, active at 37°C Stutzerimonas stutzeri
3.1.1.75 15 37 inactive at 15°C, active at 37°C Ralstonia pickettii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 5.4
-
-
 Cupriavidus sp. T1
3.1.1.75 5.4
-
-
 Ralstonia pickettii
3.1.1.75 7
-
 assay at Comamonas testosteroni
3.1.1.75 7
-
 assay at Marinobacter algicola
3.1.1.75 7
-
 assay at Stutzerimonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.75 5.4 7 reduced activity at pH 5.4 Comamonas testosteroni
3.1.1.75 5.4 7 inactive at pH 5.4, active at pH 7.0 Stutzerimonas stutzeri