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Literature summary extracted from

  • Chen, P.; Zhu, Y.; Men, Y.; Zeng, Y.; Sun, Y.
    Purification and characterization of a novel alginate lyase from the marine bacterium Bacillus sp. Alg07 (2018), Mar. Drugs, 16, 86.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.3 NaCl the optimal NaCl concentration for enzyme activity is 200 mM, and no activity is detected in the absence of NaCl Bacillus weihaiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Al3+ about 5% residual activity at 2 mM Bacillus weihaiensis
4.2.2.3 Ba2+ about 55% residual activity at 2 mM Bacillus weihaiensis
4.2.2.3 Cu2+ about 20% residual activity at 2 mM Bacillus weihaiensis
4.2.2.3 EDTA about 60% residual activity at 2 mM Bacillus weihaiensis
4.2.2.3 Fe2+ about 10% residual activity at 2 mM Bacillus weihaiensis
4.2.2.3 Fe3+ about 3% residual activity at 2 mM Bacillus weihaiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ca2+ about 215% activity at 2 mM Bacillus weihaiensis
4.2.2.3 Co2+ about 110% activity at 2 mM Bacillus weihaiensis
4.2.2.3 Mg2+ about 300% activity at 2 mM Bacillus weihaiensis
4.2.2.3 Mn2+ about 120% activity at 2 mM Bacillus weihaiensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Bacillus weihaiensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 Source 15Q column chromatography Bacillus weihaiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 additional information no activity with pectin, hyaluronan, chitin, or agar Bacillus weihaiensis ?
-
-
4.2.2.3 poly(alpha-(1->4)-L-guluronic acid) 10.5% activity compared to sodium alginate Bacillus weihaiensis disaccharides + trisaccharides + tetrasaccharides
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronic acid) 87.2% activity compared to sodium alginate Bacillus weihaiensis disaccharides + trisaccharides + tetrasaccharides
-
 ?
4.2.2.3 sodium alginate 100% activity Bacillus weihaiensis disaccharides + trisaccharides + tetrasaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 60000, SDS-PAGE Bacillus weihaiensis

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 AlgA
-
 Bacillus weihaiensis
4.2.2.3 polyM-specific alginate lyase
-
 Bacillus weihaiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 40
-
-
 Bacillus weihaiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 25 45 the enzyme shows more than 70% activity at 25-45°C Bacillus weihaiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40 50 the enzyme activity negligibly changes after 24 h of incubation at 40°C. The half-life of the enzyme is approximately 3 h under 45°C and 0.75 h under 50°C Bacillus weihaiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.3 474.6
-
 poly(beta-(1->4)-D-mannuronic acid) at pH 7.5 and 40°C Bacillus weihaiensis
4.2.2.3 911.7
-
 sodium alginate at pH 7.5 and 40°C Bacillus weihaiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.5
-
-
 Bacillus weihaiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 6 8.5 the enzyme shows more than 50% activity at pH 6.0-8.5 Bacillus weihaiensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 5 10 the enzyme presents the highest stability (more than 80% activity) for an extended period over a pH range of 5.0-10.0 Bacillus weihaiensis