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Literature summary extracted from

  • Meng, D.; Jiang, W.; Li, J.; Huang, L.; Zhai, L.; Zhang, L.; Guan, Z.; Cai, Y.; Liao, X.
    An alkaline phosphatase from Bacillus amyloliquefaciens YP6 of new application in biodegradation of five broad-spectrum organophosphorus pesticides (2019), J. Environ. Sci. Health B, 54, 336-343.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.1 degradation the strain is capable of detoxifying or degrading chlorpyrifos, and enzyme AP3 takes part in degradation of organophosphorus pesticides chlorpyrifos, dichlorvos, dipterex, phoxim, and triazophos Bacillus amyloliquefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 expression in Escherichia coli Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Ba2+ 10 mM, 56.3% of initial activity Bacillus amyloliquefaciens
3.1.3.1 Co2+ 10 mM, 71.3% of initial activity Bacillus amyloliquefaciens
3.1.3.1 EDTA
-
 Bacillus amyloliquefaciens
3.1.3.1 Fe2+ 10 mM, 21.5% of initial activity Bacillus amyloliquefaciens
3.1.3.1 L-cysteine
-
 Bacillus amyloliquefaciens
3.1.3.1 Mn2+ 10 mM, 56.3% of initial activity Bacillus amyloliquefaciens
3.1.3.1 Ni2+ 10 mM, 85.7% of initial activity Bacillus amyloliquefaciens
3.1.3.1 Zn2+ activating up to 0.5 mM, inhibitory at 1-10 mM Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 12.2
-
 4-nitrophenyl phosphate pH 10.3, 40°C Bacillus amyloliquefaciens
3.1.3.1 101.2
-
 Phenyl phosphate pH 10.3, 40°C Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Ca2+ 10 mM, 120.5% of initial activity Bacillus amyloliquefaciens
3.1.3.1 Cu2+ 10 mM, 118.1% of initial activity Bacillus amyloliquefaciens
3.1.3.1 Mg2+ 10 mM, 130.7% of initial activity Bacillus amyloliquefaciens
3.1.3.1 Zn2+ activating up to 0.5 mM, inhibitory at 1-10 mM Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Bacillus amyloliquefaciens
-
-
-
3.1.3.1 Bacillus amyloliquefaciens YP6
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Bacillus amyloliquefaciens 4-nitrophenol + phosphate
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Bacillus amyloliquefaciens YP6 4-nitrophenol + phosphate
-
 ?
3.1.3.1 chlorpyrifos + H2O
-
 Bacillus amyloliquefaciens ? + phosphate
-
 ?
3.1.3.1 dichlorvos + H2O
-
 Bacillus amyloliquefaciens ? + phosphate
-
 ?
3.1.3.1 dichlorvos + H2O
-
 Bacillus amyloliquefaciens YP6 ? + phosphate
-
 ?
3.1.3.1 dipterex + H2O
-
 Bacillus amyloliquefaciens ? + phosphate
-
 ?
3.1.3.1 phenyl phosphate + H2O
-
 Bacillus amyloliquefaciens phenol + phosphate
-
 ?
3.1.3.1 phenyl phosphate + H2O
-
 Bacillus amyloliquefaciens YP6 phenol + phosphate
-
 ?
3.1.3.1 phoxim + H2O
-
 Bacillus amyloliquefaciens ? + phosphate
-
 ?
3.1.3.1 phoxim + H2O
-
 Bacillus amyloliquefaciens YP6 ? + phosphate
-
 ?
3.1.3.1 triazophos + H2O
-
 Bacillus amyloliquefaciens ? + phosphate
-
 ?
3.1.3.1 triazophos + H2O
-
 Bacillus amyloliquefaciens YP6 ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 49600, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 AP3
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 40
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 4 55 1 h, more than 55% residual activity Bacillus amyloliquefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.1 additional information
-
 4-nitrophenyl phosphate turnover number is 3300000/s, pH 10.3, 40°C Bacillus amyloliquefaciens
3.1.3.1 additional information
-
 Phenyl phosphate turnover number is 2540000/s, pH 10.3, 40°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 10.3
-
-
 Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 8 11 1 h, more than 50% residual activity Bacillus amyloliquefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.1 25000
-
 Phenyl phosphate pH 10.3, 40°C Bacillus amyloliquefaciens
3.1.3.1 270000
-
 4-nitrophenyl phosphate pH 10.3, 40°C Bacillus amyloliquefaciens