Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jurkowitz, M.S.; Azad, A.K.; Monsma, P.C.; Keiser, T.L.; Kanyo, J.; Lam, T.T.; Bell, C.E.; Schlesinger, L.S.
    Mycobacterium tuberculosis encodes a YhhN family membrane protein with lysoplasmalogenase activity that protects against toxic host lysolipids (2022), J. Biol. Chem., 298, 101849.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.2 expression in Mycobacterium smegmatis Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.2 0.0769
-
 choline lysoplasmalogen pH 7.0, 37°C Mycobacterium tuberculosis
3.3.2.2 0.0894
-
 ethanolamine lysoplasmalogen pH 7.0, 37°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.2 Mycobacterium tuberculosis P9WG51
-
-
3.3.2.2 Mycobacterium tuberculosis H37Rv P9WG51
-
-

Storage Stability

EC Number Storage Stability Organism
3.3.2.2 -20°C, complete loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.2 choline lysoplasmalogen + H2O
-
 Mycobacterium tuberculosis ? + glycerophospho-choline
-
 ?
3.3.2.2 choline lysoplasmalogen + H2O
-
 Mycobacterium tuberculosis H37Rv ? + glycerophospho-choline
-
 ?
3.3.2.2 ethanolamine lysoplasmalogen + H2O
-
 Mycobacterium tuberculosis ? + glycerophospho-ethanolamine
-
 ?
3.3.2.2 ethanolamine lysoplasmalogen + H2O
-
 Mycobacterium tuberculosis H37Rv ? + glycerophospho-ethanolamine
-
 ?
3.3.2.2 additional information enzyme specifically hydrolyzes the vinyl ether bonds of ethanolamine lysoplasmalogen and choline lysoplasmalogen and has no activity toward the ester linkage of lysophosphatidylcholie or lysophosphatidylethanolamine Mycobacterium tuberculosis ?
-
-
3.3.2.2 additional information enzyme specifically hydrolyzes the vinyl ether bonds of ethanolamine lysoplasmalogen and choline lysoplasmalogen and has no activity toward the ester linkage of lysophosphatidylcholie or lysophosphatidylethanolamine Mycobacterium tuberculosis H37Rv ?
-
-

Subunits

EC Number Subunits Comment Organism
3.3.2.2 ? x * 23000 and x * 38000, SDS-PAGE of recombinant protein, x * 27373, calculated from sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.3.2.2 Rv1401
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.3.2.2 100
-
 5 min, complete loss of activity Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.2 6.6 7.2
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.3.2.2 physiological function overexpression of YhhN in Mycobacterium smegmatis cells protexts spheroplast from membrane disruption/lysis by choline lysoplasmalogen. Overexpression of full-length YhhN in Mycobacterium smegmatis increases its survival within human macrophages by 2.6fold Mycobacterium tuberculosis