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Literature summary extracted from

  • Ristinmaa, A.S.; Coleman, T.; Cesar, L.; Langborg Weinmann, A.; Mazurkewich, S.; Braenden, G.; Hasani, M.; Larsbrink, J.
    Structural diversity and substrate preferences of three tannase enzymes encoded by the anaerobic bacterium Clostridium butyricum (2022), J. Biol. Chem., 298, 101758.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.20 expression in Escherichia coli Clostridium butyricum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 1 2 propyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 2 3 beta-glucogallin isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 2 2.2 propyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 2 3.4 methyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 3.5
-
 beta-glucogallin isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 7.2
-
 propyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 9
-
 beta-glucogallin isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 10.8
-
 methyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 24.3
-
 methyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Clostridium butyricum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 beta-glucogallin + H2O
-
 Clostridium butyricum gallate + D-glucose
-
 ?
3.1.1.20 hexyl gallate + H2O
-
 Clostridium butyricum gallate + hexanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Clostridium butyricum gallate + methanol
-
 ?
3.1.1.20 oak bark extract + H2O
-
 Clostridium butyricum ?
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Clostridium butyricum gallate + propanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 65000, isoform Tan1, x * 55000, isoform Tan2, SDS-PAGE Clostridium butyricum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 K670DRAFT_03878 locus tag Clostridium butyricum
3.1.1.20 K670DRAFT_04016 locus tag Clostridium butyricum
3.1.1.20 K670DRAFT_04079 locus tag Clostridium butyricum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.20 15.9
-
 propyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 26.9
-
 methyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 32
-
 propyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 45.9
-
 methyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 136
-
 propyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 187
-
 methyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 209
-
 beta-glucogallin isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 213
-
 beta-glucogallin isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 459
-
 beta-glucogallin isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 6 7 isoforms Tan1, Tan2 Clostridium butyricum
3.1.1.20 7
-
 isoform Tan3 Clostridium butyricum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.20 1.6
-
 methyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 2.2
-
 propyl gallate isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 2.4
-
 methyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 2.6
-
 propyl gallate isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 6.2
-
 propyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 8
-
 methyl gallate isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 10.2
-
 beta-glucogallin isoform Tan2, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 52.9
-
 beta-glucogallin isoform Tan3, 37°C, pH not specified in the publication Clostridium butyricum
3.1.1.20 72.8
-
 beta-glucogallin isoform Tan1, 37°C, pH not specified in the publication Clostridium butyricum