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Literature summary extracted from

  • Liu, J.; Liu, Z.; Jiang, C.; Mao, X.
    Biochemical characterization and substrate degradation mode of a novel beta-agarase from Catenovulum agarivorans (2019), J. Agric. Food Chem., 67, 10373-10379.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.158 synthesis the enzyme (CaLJ96) has potential for the manufacture of bioactive agarooligosaccharides Catenovulum agarivorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.158 expression in Escherichia coli BL21(DE3) Catenovulum agarivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.158 Ba2+ 10 mM, inhibition to 96.5% of control Catenovulum agarivorans
3.2.1.158 Co2+ 10 mM, inhibition to 28.9% of control Catenovulum agarivorans
3.2.1.158 Cu2+ 10 mM, inhibition to 3.3% of control Catenovulum agarivorans
3.2.1.158 Fe3+ 10 mM, inhibition to 1.2% of control Catenovulum agarivorans
3.2.1.158 K+ 10 mM, inhibition to 58.4% of control Catenovulum agarivorans
3.2.1.158 Mg2+ 10 mM, inhibition to 46.0% of control Catenovulum agarivorans
3.2.1.158 Mn2+ 10 mM, inhibition to 97.8% of control Catenovulum agarivorans
3.2.1.158 Na+ 10 mM, inhibition to 58.1% of control Catenovulum agarivorans
3.2.1.158 Na2EDTA 10 mM, complete inhibition Catenovulum agarivorans
3.2.1.158 Ni2+ 10 mM, complete inhibition Catenovulum agarivorans
3.2.1.158 SDS 10 mM, inhibition to 11.2% of control Catenovulum agarivorans
3.2.1.158 Zn2+ 10 mM, complete inhibition Catenovulum agarivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.158 additional information
-
 agarose KM: 0.135 mg/ml, pH 7.0, 37°C Catenovulum agarivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.158 Ca2+ 10 mM, activation to 116.7% of control Catenovulum agarivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.158 200000
-
 SDS-PAGE Catenovulum agarivorans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.158 Catenovulum agarivorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.158
-
 Catenovulum agarivorans

Storage Stability

EC Number Storage Stability Organism
3.2.1.158 4°C, 28 d, more than 70% of initial activity is retained Catenovulum agarivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.158 agarohexaose + H2O
-
 Catenovulum agarivorans agarobiose + agarotetraose
-
 ?
3.2.1.158 agaropentaose + H2O
-
 Catenovulum agarivorans agarobiose + agarotriose
-
 ?
3.2.1.158 agarose + H2O agarotetraose is the dominant product. No actiovity with: agarobiose, agarotriose and agarotetraose Catenovulum agarivorans agarobiose + agarotetraose + agarohexaose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.158 CaLJ96
-
 Catenovulum agarivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.158 37
-
-
 Catenovulum agarivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.158 30 50 more than 80% of the highest enzyme activity is maintained at 30-50°C, and the activity at 20°C is 12.3% of that at 37°C Catenovulum agarivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.158 20 30 1 h, stable Catenovulum agarivorans
3.2.1.158 37
-
 1 h, complete loss of activity at temperature higher than 37°C Catenovulum agarivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.158 2.4
-
 agarose pH 7.0, 37°C Catenovulum agarivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.158 7
-
-
 Catenovulum agarivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.158 3 5 the activity is less than 10% of that at pH 7.0 Catenovulum agarivorans
3.2.1.158 7 10 more than 80% of the highest enzyme activity is retained at pH 7.0-10.0 Catenovulum agarivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.158 additional information
-
 agarose kcat/Km: 17.78 ml/mg*s, pH 7.0, 37°C Catenovulum agarivorans