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Literature summary extracted from

  • Paul, A.; Mishra, A.; Surolia, A.; Vijayan, M.
    Structural studies on M. tuberculosis argininosuccinate lyase and its liganded complex Insights into catalytic mechanism (2019), IUBMB Life, 71, 643-652.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.1 microbatch under oil method, apoenzyme using 100 mM Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350, enzyme bound to substrate using 2.1 M DL-malic acid pH 7.0 and 10% (v/v) ethylene glycol Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.1 2-(Nomega-L-arginino)succinate Mycobacterium tuberculosis
-
 fumarate + L-arginine
-
 r
4.3.2.1 2-(Nomega-L-arginino)succinate Mycobacterium tuberculosis H37Rv
-
 fumarate + L-arginine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.1 Mycobacterium tuberculosis P9WPY7
-
-
4.3.2.1 Mycobacterium tuberculosis H37Rv P9WPY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.1 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.1 2-(Nomega-L-arginino)succinate
-
 Mycobacterium tuberculosis fumarate + L-arginine
-
 r
4.3.2.1 2-(Nomega-L-arginino)succinate
-
 Mycobacterium tuberculosis H37Rv fumarate + L-arginine
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.2.1 homotetramer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.3.2.1 ASL
-
 Mycobacterium tuberculosis