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Literature summary extracted from

  • He, D.; Du, Z.; Xu, H.; Bao, X.
    Chl1, an ATP-dependent DNA helicase, inhibits DNA RNA hybrids formation at DSB sites to maintain genome stability in S. pombe (2022), Int. J. Mol. Sci., 23, 6631.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.3 recobinant expression of FLAG-tagged Chl1 driven by its natural promoter Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.3 additional information generation of an enzyme-deletion mutant strain. The chl1DELTA strain is less sensitive to DNA damaging agent. In the chl1DELTA strains, homologous recombination (HR) and nonhomologous end-joining (NHEJ) are compromised significantly around the cleavage site, and mutation of RNase H further reduces DNA repair efficiency via these two methods, suggesting that Chl1 promotes DNA damage repair (DDR) by hampering the accumulation of DNA:RNA hybrids at DSB sites. Following DSB induction, Chl1 deletion results in a decreased ratio of gene conversion (GC) (62.8%) compared with the wild-type (68.3%). In the absence of RNase H genes, the GC ratio is further reduced in a chl1DELTA background, which suggests that the elevated level of DNA:RNA structures in chl1D mutants suppresses HR efficiency. The plasmid rejoining ratio in the mutant chl1DELTA strain is markedly lower than that in the wild-type, and deletion of both RNase H1 and RNase H201 in the chl1DELTA strain further reduces NHEJ efficiency Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.3 chromatin distribution of Chl1 at double-strand DNA break (DSB) sites Schizosaccharomyces pombe 785
-
5.6.2.3 nucleus
-
 Schizosaccharomyces pombe 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.3 Mg2+ required Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.3 Schizosaccharomyces pombe O14147
-
-
5.6.2.3 Schizosaccharomyces pombe 972 O14147
-
-
5.6.2.3 Schizosaccharomyces pombe ATCC 24843 O14147
-
-

Synonyms

EC Number Synonyms Comment Organism
5.6.2.3 ATP-dependent DNA helicase
-
 Schizosaccharomyces pombe
5.6.2.3 CHL1
-
 Schizosaccharomyces pombe
5.6.2.3 ChlR1/DDX11
-
 Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.3 7.5
-
 assay at Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.3 ATP dependent on Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
5.6.2.3 malfunction in the chl1DELTA strains, homologous recombination (HR) and nonhomologous end-joining (NHEJ) are compromised significantly around the cleavage site, and mutation of RNase H further reduces DNA repair efficiency via these two methods, suggesting that Chl1 promotes DNA damage repair (DDR) by hampering the accumulation of DNA:RNA hybrids at DSB sites. Following DSB induction, Chl1 deletion results in a decreased ratio of gene conversion (GC) (62.8%) compared with the wild-type (68.3%). In the absence of RNase H genes, the GC ratio is further reduced in a chl1DELTA background, which suggests that the elevated level of DNA:RNA structures in chl1D mutants suppresses HR efficiency. The plasmid rejoining ratio in the mutant chl1DELTA strain is markedly lower than that in the wild-type, and deletion of both RNase H1 and RNase H201 in the chl1DELTA strain further reduces NHEJ efficiency Schizosaccharomyces pombe
5.6.2.3 additional information enzyme interaction partner identification by tandem mass spectrometry analysis of purified enzyme-protein complexes, and interaction analysis between Chl1 and Rpc37 in vivo, using generated strains expressing Chl1-Flag and Rpc37-Myc at their native chromosomal loci, followed by immunoprecipitation of Chl1-Flag captured endogenous Rpc37 from cell extracts and yeast two-hybrid (Y2H) analysis. Chl1 interacts with the subunit Rpc37 of RNA Pol III. Chl1 does not interact with Mcl1 or Eso1 Schizosaccharomyces pombe
5.6.2.3 physiological function helicase Chl1 inhibits DNA:RNA hybrid accumulation at HO-induced double-strand DNA breaks (DSBs) in Schizosaccharomyces pombe, determined by immunoaffinity analysis. Chl1 promotes DNA damage repair (DDR) by hampering the accumulation of DNA:RNA hybrids at DSB sites. DNA:RNA hybrids reduce HR efficiency by suppressing Rad52 enrichment around HO cleavage sites. Chl1 physically interacts with Rpc37, an RNA Pol III-specific subunit that recruits Chl1 to DSB sites, suggesting a role for Rpc5, which participates in DDR through interaction with Chl1. Chl1 is an essential factor in DDR. In addition, Chl1 plays a crucial role in sister chromatid cohesion establishment. The recruitment of Chl1 to the replisome during replication is indispensable for sister chromatid cohesion Schizosaccharomyces pombe