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Literature summary extracted from

  • Zhang, J.; Lu, F.; Li, M.
    Identification and investigation of the effects of N-acetylmuramoyl-L-alanine amidase in Bacillus amyloliquefaciens for the cell lysis and heterologous protein production (2024), Int. J. Biol. Macromol., 256, 128468.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 six N-acetylmuramic acid-L-alanine amidase genes are identified in Bacillus amyloliquefaciens strain TCCC11018, transcriptome analysis and quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain EC135 Bacillus amyloliquefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.28 additional information construction of CRISPR-nCa9 system in Bacillus amyloliquefaciens single or tandem knockout of the six N-acetylmuramic acid-L-alanine amidase genes identified in Bacillus amyloliquefaciens strain TCCC11018 revealing that modification of LytH and CwlC1 can slow down cell lysis. The autolysis rate of the mutant strain BA DELTAytH-cwlC1 decreases by 4.83%, and the amylase activity reaches 176 U/ml, which is 76.04% higher than that of the control strain, growth profiles, overview Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Bacillus amyloliquefaciens A0AAP3YI41 Bacillus velezensis
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3.5.1.28 Bacillus amyloliquefaciens TCCC11018 A0AAP3YI41 Bacillus velezensis
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Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 Cell wall hydrolase
-
 Bacillus amyloliquefaciens
3.5.1.28 L-Ala-D-Glu endopeptidase UniProt Bacillus amyloliquefaciens
3.5.1.28 lytH
-
 Bacillus amyloliquefaciens
3.5.1.28 N-acetylmuranyl-L-alanine amidase
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.28 70
-
 assay at Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 6
-
 assay at Bacillus amyloliquefaciens

General Information

EC Number General Information Comment Organism
3.5.1.28 malfunction single or multiple deletion of the lytH and cwlC1 genes blocks cell lysis and improves heterologous protein acid-stable Bacillus licheniformis alpha amylase (BLA) production Bacillus amyloliquefaciens
3.5.1.28 physiological function the self-structural degradation process that bacteria undergo at the end of their life cycle autolysis, involving autolysins such as peptidoglycan hydrolases, which catalyze the hydrolysis of the cell wall peptidoglycan layer Bacillus amyloliquefaciens