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Literature summary extracted from
- Biochemical characterization of a GH19 chitinase from Streptomyces alfalfae and its applications in crystalline chitin conversion and biocontrol (2021), Int. J. Biol. Macromol., 167, 193-201.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | dithiothreitol | 1 mM, 118.81% of initial activity | Streptomyces alfalfae |  |
| 3.2.1.14 | EDTA | 1 mM, 103.81% of initial activity | Streptomyces alfalfae | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | degradation | in combination with N-acetyl hexosaminidase SaHEX, the enzyme yields a conversion rate of 95.2% from chitin powder to N-acetyl-D-glucosamine in 8 h and a product purity of >98.5% | Streptomyces alfalfae |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | expression in Escherichia coli | Streptomyces alfalfae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | AgCl | 1 mM, 36.45% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | CuCl2 | 1 mM, 70.95% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | HgCl2 | 1 mM, no activity | Streptomyces alfalfae | |
| 3.2.1.14 | MnCl2 | 1 mM, 91.67% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | NaCl | 1 mM, 89.05% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | NiCl2 | 1 mM, 69.04% of initial activity | Streptomyces alfalfae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | CaCl2 | 1 mM, 116.67% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | CoCl2 | 1 mM, 184.13% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | FeCl2 | 1 mM, 125.76% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | FeCl3 | 1 mM, 115.43% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | KCl | 1 mM, 107.86% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | MgCl2 | 1 mM, 110.52% of initial activity | Streptomyces alfalfae | |
| 3.2.1.14 | ZnCl2 | 1 mM, 111.91% of initial activity | Streptomyces alfalfae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Streptomyces alfalfae | - |
- |
- |
| 3.2.1.14 | Streptomyces alfalfae ACCC 40021 | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 8.7 | - |
substrate beta chitin, pH 8, 45°C | Streptomyces alfalfae |
| 3.2.1.14 | 28.4 | - |
substrate colloidal chitin, pH 8, 45°C | Streptomyces alfalfae |
| 3.2.1.14 | 286.6 | - |
substrate glycol chitin, pH 8, 45°C | Streptomyces alfalfae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | beta-chitin + H2O | - |
Streptomyces alfalfae | ? | - |
? | |
| 3.2.1.14 | beta-chitin + H2O | - |
Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
| 3.2.1.14 | chitooligosaccharides + H2O | degree of polymerization 3-6 | Streptomyces alfalfae | ? | - |
? | |
| 3.2.1.14 | chitooligosaccharides + H2O | degree of polymerization 3-6 | Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
| 3.2.1.14 | colloidal chitin + H2O | - |
Streptomyces alfalfae | ? | - |
? | |
| 3.2.1.14 | colloidal chitin + H2O | - |
Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
| 3.2.1.14 | glycol chitin + H2O | best substrate | Streptomyces alfalfae | ? | - |
? | |
| 3.2.1.14 | glycol chitin + H2O | best substrate | Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x* 29000, calculated from sequence, x * 33000, SDS-PAGE of recombinant protein | Streptomyces alfalfae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | A7J05_RS04860 | locus name | Streptomyces alfalfae |
| 3.2.1.14 | ChiB | - |
Streptomyces alfalfae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 45 | - |
- |
Streptomyces alfalfae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 30 | 60 | - |
Streptomyces alfalfae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 55 | - |
2 h, 90% residual activity | Streptomyces alfalfae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 8 | - |
- |
Streptomyces alfalfae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | 11 | - |
Streptomyces alfalfae |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.14 | Streptomyces alfalfae | calculated from sequence | - |
6.9 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | physiological function | the enzyme strongly inhibits the growth of tested pathogenic fungi | Streptomyces alfalfae |
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