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Literature summary extracted from

  • Liu, Y.; Wu, Z.; Zeng, X.; Weng, P.; Zhang, X.; Wang, C.
    A novel cold-adapted phospho-beta-galactosidase from Bacillus velezensis and its potential application for lactose hydrolysis in milk (2021), Int. J. Biol. Macromol., 166, 760-770.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.85 SDS 5 mM, activation to 149% of control Bacillus amyloliquefaciens

Application

EC Number Application Comment Organism
3.2.1.85 food industry the enzyme is a promising industrial biocatalyst for efficient lactose hydrolysis in milk Bacillus amyloliquefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.85 expression in Escherichia coli BL21(DE3) Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.85 Ag+ 5 mM, inhibition to 93% of control Bacillus amyloliquefaciens
3.2.1.85 Ba2+ 5 mM, inhibition to 84% of control Bacillus amyloliquefaciens
3.2.1.85 carboxymethylcellulose 10%, complete inactivation Bacillus amyloliquefaciens
3.2.1.85 Co2+ 5 mM, inhibition to 25% of control Bacillus amyloliquefaciens
3.2.1.85 Cu2+ 5 mM, complete inactivation Bacillus amyloliquefaciens
3.2.1.85 EDTA 10%, inhibition to 24% of control Bacillus amyloliquefaciens
3.2.1.85 ethanol 10%, inhibition to 82% of control Bacillus amyloliquefaciens
3.2.1.85 Fe2+ 5 mM, inhibition to 3% of control Bacillus amyloliquefaciens
3.2.1.85 Fe3+ 5 mM, inhibition to 15% of control Bacillus amyloliquefaciens
3.2.1.85 Isopropanol 10%, inhibition to 74% of control Bacillus amyloliquefaciens
3.2.1.85 Mg2+ 5 mM, inhibition to 84% of control Bacillus amyloliquefaciens
3.2.1.85 Mn2+ 5 mM, inhibition to 28% of control Bacillus amyloliquefaciens
3.2.1.85 Triton X-100 10%, inhibition to 57% of control Bacillus amyloliquefaciens
3.2.1.85 Tween-40 10%, inhibition to 96% of control Bacillus amyloliquefaciens
3.2.1.85 Tween-80 10%, complete inactivation Bacillus amyloliquefaciens
3.2.1.85 Urea 10%, inhibition to 94% of control Bacillus amyloliquefaciens
3.2.1.85 Zn2+ 5 mM, inhibition to 68% of control Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 1.95
-
 lactose pH 8, 45°C Bacillus amyloliquefaciens
3.2.1.85 2.05
-
 lactose pH 8, 10°C Bacillus amyloliquefaciens
3.2.1.85 2.3 6 lactose pH 8, 4°C Bacillus amyloliquefaciens
3.2.1.85 18.64
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 45°C Bacillus amyloliquefaciens
3.2.1.85 21.53
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 10°C Bacillus amyloliquefaciens
3.2.1.85 23.68
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 4°C Bacillus amyloliquefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.85 intracellular
-
 Bacillus amyloliquefaciens 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.85 K+ 5 mM, activation to 102% of control Bacillus amyloliquefaciens
3.2.1.85 Na+ slight activation Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.85 53987
-
 calculated Bacillus amyloliquefaciens
3.2.1.85 55000
-
 SDS-PAGE Bacillus amyloliquefaciens
3.2.1.85 113000
-
 gel filtration Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Bacillus amyloliquefaciens A0A7S8BFT0
-
-
3.2.1.85 Bacillus amyloliquefaciens SW5 A0A7S8BFT0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85
-
 Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 2-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Bacillus amyloliquefaciens 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.85 2-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Bacillus amyloliquefaciens SW5 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.85 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O 80% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens 2-nitrophenol + beta-D-galactose 6-phosphate
-
 ?
3.2.1.85 2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O 80% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens SW5 2-nitrophenol + beta-D-galactose 6-phosphate
-
 ?
3.2.1.85 4-nitrophenyl-beta-D-galactopyranoside + H2O 147% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.85 4-nitrophenyl-beta-D-galactopyranoside + H2O 147% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens SW5 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.85 lactose + H2O
-
 Bacillus amyloliquefaciens D-glucose + D-galactose
-
 ?
3.2.1.85 lactose + H2O
-
 Bacillus amyloliquefaciens SW5 D-glucose + D-galactose
-
 ?
3.2.1.85 additional information the enzyme (BsGal1332) displays relative strict substrate specificity for galactosyl compounds compared with the other phospho-beta-galactosidases Bacillus amyloliquefaciens ?
-
-
3.2.1.85 additional information the enzyme (BsGal1332) displays relative strict substrate specificity for galactosyl compounds compared with the other phospho-beta-galactosidases Bacillus amyloliquefaciens SW5 ?
-
-

Subunits

EC Number Subunits Comment Organism
3.2.1.85 homodimer 2 * 55000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 BsGal1332
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.85 40
-
 substrate: lactose Bacillus amyloliquefaciens
3.2.1.85 50
-
 substrate: 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.85
-
 60 0°C: about 75% of maximal activity, 60°C: about 80% of maximal activity, substrate: 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens
3.2.1.85
-
 60 0°C: about 85% of maximal activity, 60°C: about 70% of maximal activity, substrate: lactose Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.85 40
-
 stable below Bacillus amyloliquefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.85 117.55
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 4°C Bacillus amyloliquefaciens
3.2.1.85 124.45
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 10°C Bacillus amyloliquefaciens
3.2.1.85 134.45
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 45°C Bacillus amyloliquefaciens
3.2.1.85 155.61
-
 lactose pH 8, 4°C Bacillus amyloliquefaciens
3.2.1.85 171.74
-
 lactose pH 8, 10°C Bacillus amyloliquefaciens
3.2.1.85 198.24
-
 lactose pH 8, 45°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.85 8
-
 substrate: 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.85 5 10 pH 5.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity, substrate: 2-nitrophenyl-beta-D-galactopyranoside Bacillus amyloliquefaciens
3.2.1.85 5 10 pH 5.0: about 50% of maximal activity, pH 10.0: about 50% of maximal activity, substrate: lactose Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.85 6 7 stable Bacillus amyloliquefaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.85 Bacillus amyloliquefaciens calculated
-
 5.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.85 4.96
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 4°C Bacillus amyloliquefaciens
3.2.1.85 5.78
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 10°C Bacillus amyloliquefaciens
3.2.1.85 7.21
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8, 45°C Bacillus amyloliquefaciens
3.2.1.85 65.94
-
 lactose pH 8, 4°C Bacillus amyloliquefaciens
3.2.1.85 83.78
-
 lactose pH 8, 10°C Bacillus amyloliquefaciens
3.2.1.85 101.66
-
 lactose pH 8, 45°C Bacillus amyloliquefaciens