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Literature summary extracted from

  • Luo, S.; Qin, Z.; Chen, Q.; Fan, L.; Jiang, L.; Zhao, L.
    High level production of a Bacillus amlyoliquefaciens chitosanase in Pichia pastoris suitable for chitooligosaccharides preparation (2020), Int. J. Biol. Macromol., 149, 1034-1041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expression in Pichia pastoris Bacillus amyloliquefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Bacillus amyloliquefaciens
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus amyloliquefaciens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.132 glycoprotein
-
 Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 2380.5
-
 pH 6.5, 55°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitopentaose + H2O
-
 Bacillus amyloliquefaciens chitobiose + chitotriose
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus amyloliquefaciens chitobiose + chitotriose
-
 ?
3.2.1.132 chitotetraose + H2O
-
 Bacillus amyloliquefaciens 2 chitobiose
-
 ?
3.2.1.132 additional information enzyme breaks the beta-1,4-glycosidic bonds in chitosan, whereas the beta-1,4-glycosidic bonds in chitin and cellulose cannot be hydrolyzed. Chitobiose and chitotriose are very poor substrates Bacillus amyloliquefaciens ?
-
-

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 29000, SDS-PAGE of deglycosylated enzyme, X * 44300, SDS-PAGE of recombinant enzyme Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 25
-
 30 min, more than 80% residual activity Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6.5
-
 acetate buffer Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 7
-
 strong decrease in activity Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 4.5 8.5
-
 Bacillus amyloliquefaciens