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Literature summary extracted from

  • Zhou, W.; Jiang, H.; Liang, X.; Qiu, Y.; Wang, L.; Mao, X.
    Discovery and characterization of a novel alpha-L-fucosidase from the marine-derived Flavobacterium algicola and its application in 2-fucosyllactose production (2022), Food Chem., 369, 130942.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.51 synthesis the enzyme could be a promising tool to prepare 2'-fucosyllactose and other novel glycosides. The enzyme is capable of transferring the fucosyl residue to other glycosyl receptors with the generation of novel fucosylated compounds Flavobacterium algicola

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 expression in Escherichia coli BL21 (DE3) Flavobacterium algicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 Ba2+ 10 mM, about 10% loss of activity Flavobacterium algicola
3.2.1.51 Ca2+ 10 mM, about 30% loss of activity Flavobacterium algicola
3.2.1.51 Cu2+ 10 mM, about 95% loss of activity Flavobacterium algicola
3.2.1.51 K+ 10 mM, about 20% loss of activity Flavobacterium algicola
3.2.1.51 Mg2+ 10 mM, about 25% loss of activity Flavobacterium algicola
3.2.1.51 Na2EDTA 10 mM, about 10% loss of activity Flavobacterium algicola
3.2.1.51 Ni2+ 10 mM, about 20% loss of activity Flavobacterium algicola
3.2.1.51 SDS 10 mM, about 55% loss of activity Flavobacterium algicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 1.043
-
 4-nitrophenyl alpha-L-fucopyranoside pH 6.0, 25°C Flavobacterium algicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 54000
-
 SDS-PAGE Flavobacterium algicola
3.2.1.51 54300
-
 calculated Flavobacterium algicola

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Flavobacterium algicola A0A8F9R4B3
-
-
3.2.1.51 Flavobacterium algicola 12076 A0A8F9R4B3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Flavobacterium algicola

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 20°C, 12 h, 89.66% of the enzyme activity is retained Flavobacterium algicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 2'-fucosyllactose + H2O
-
 Flavobacterium algicola alpha-L-fucose + lactose
-
 ?
3.2.1.51 2'-fucosyllactose + H2O
-
 Flavobacterium algicola 12076 alpha-L-fucose + lactose
-
 ?
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Flavobacterium algicola 4-nitrophenol + alpha-L-fucopyranose
-
 ?
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Flavobacterium algicola 12076 4-nitrophenol + alpha-L-fucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 ? x * 54000, SDS-PAGE Flavobacterium algicola

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 OUC-Jdch16
-
 Flavobacterium algicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 25
-
 hydrolysis of 4-nitrophenyl alpha-L-fucopyranoside Flavobacterium algicola

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.51 20 35 20°C: about 50% of maximal activity, 35°C: about 50% of maximal activity, hydrolysis of 4-nitrophenyl alpha-L-fucopyranoside Flavobacterium algicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 25
-
 12 h, less than 70% of the enzyme activity is retained Flavobacterium algicola
3.2.1.51 35
-
 12, no residual activity Flavobacterium algicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 6
-
 hydrolysis of 4-nitrophenyl alpha-L-fucopyranoside Flavobacterium algicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 5 7 pH 5.0: about 75% of maximal activity, pH 7.0: about30 % of maximal activity, hydrolysis of 4-nitrophenyl alpha-L-fucopyranoside Flavobacterium algicola

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 4
-
 12 h, citrate buffer, enzyme retains 79.71% of its initial activity Flavobacterium algicola
3.2.1.51 4 7 stable Flavobacterium algicola
3.2.1.51 8 10 low storage stability Flavobacterium algicola