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Literature summary extracted from

  • Ma, D.; Cheng, Z.; Peplowski, L.; Han, L.; Xia, Y.; Hou, X.; Guo, J.; Yin, D.; Rao, Y.; Zhou, Z.
    Insight into the broadened substrate scope of nitrile hydratase by static and dynamic structure analysis (2022), Chem. Sci., 13, 8417-8428.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.84 A129R mutation in subunit beta, mutant exhibits an expanded substrate scope, especially toward bulky nitrile substrates. The amino acid residue located at the entrance of the substrate access tunnel is a key factor in enzyme catalytic performance Pseudonocardia thermophila
4.2.1.84 M46R mutation in subunit beta, mutant exhibits an expanded substrate scope, especially toward bulky nitrile substrates. The amino acid residue located at the entrance of the substrate access tunnel is a key factor in enzyme catalytic performance Pseudonocardia thermophila
4.2.1.84 M46R/A129R mutations in subunit beta, double mutant exhibits lower activity than the two single mutants Pseudonocardia thermophila

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Pseudonocardia thermophila Q7SID2 and Q7SID3 Q7SID2 i.e. subunit alpha, Q7SID3 i.e. subunit beta
-
4.2.1.84 Pseudonocardia thermophila JCM 3095 Q7SID2 and Q7SID3 Q7SID2 i.e. subunit alpha, Q7SID3 i.e. subunit beta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 5.4
-
 wild-type, substrate thiacloprid, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 13.1
-
 wild-type, substrate 1-naphthonitrile, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 14.4
-
 wild-type, substrate pentanenitrile, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 45.8
-
 wild-type, substrate cinnamonitrile, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 66.7
-
 wild-type, substrate 3-cyanopyrazine, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 176
-
 wild-type, substrate benzonitrile, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 203
-
 wild-type, substrate 2-cyanopyrazine, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 666
-
 wild-type, substrate isobutyronitrile, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 1982
-
 wild-type, substrate hexanenitrile, pH 7.4, 25°C Pseudonocardia thermophila
4.2.1.84 2463
-
 wild-type, substrate acrylonitrile, pH 7.4, 25°C Pseudonocardia thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 1-naphthonitrile + H2O
-
 Pseudonocardia thermophila naphthalene-1-carboxamide
-
 ?
4.2.1.84 2-cyanopyrazine + H2O
-
 Pseudonocardia thermophila pyrazincarbamide
-
 ?
4.2.1.84 2-cyanopyrazine + H2O
-
 Pseudonocardia thermophila JCM 3095 pyrazincarbamide
-
 ?
4.2.1.84 3-cyanopyridine + H2O
-
 Pseudonocardia thermophila nicotinamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Pseudonocardia thermophila 2-propenoic acid amide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Pseudonocardia thermophila JCM 3095 2-propenoic acid amide
-
 ?
4.2.1.84 benzonitrile + H2O
-
 Pseudonocardia thermophila benzamide
-
 ?
4.2.1.84 cinnamonitrile + H2O
-
 Pseudonocardia thermophila (2E)-3-phenylprop-2-enamide
-
 ?
4.2.1.84 hexanenitrile + H2O
-
 Pseudonocardia thermophila hexanamide
-
 ?
4.2.1.84 hexanenitrile + H2O
-
 Pseudonocardia thermophila JCM 3095 hexanamide
-
 ?
4.2.1.84 isobutyronitrile + H2O
-
 Pseudonocardia thermophila 2-methylpropanamide
-
 ?
4.2.1.84 isobutyronitrile + H2O
-
 Pseudonocardia thermophila JCM 3095 2-methylpropanamide
-
 ?
4.2.1.84 pentanenitril + H2O
-
 Pseudonocardia thermophila pentanamide
-
 ?
4.2.1.84 pentanenitril + H2O
-
 Pseudonocardia thermophila JCM 3095 pentanamide
-
 ?
4.2.1.84 thiacloprid + H2O
-
 Pseudonocardia thermophila N-[3-[(6-chloropyridin-3-yl)methyl]-1,3-thiazolidin-2-ylidene]urea
-
 ?