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Literature summary extracted from

  • Rojas, B.E.; Hartman, M.D.; Figueroa, C.M.; Leaden, L.; Podesta, F.E.; Iglesias, A.A.
    Biochemical characterization of phosphoenolpyruvate carboxykinases from Arabidopsis thaliana (2019), Biochem. J., 476, 2939-2952.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.49 malate 50% activation at 2.6 mM, Hill coeficient 1.2. In presence of Mg2+, 50% activation at 2.1 mM, Hill coeficient 14.1 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.49 2-oxoglutarate
-
 Arabidopsis thaliana
4.1.1.49 3-phosphoglycerate
-
 Arabidopsis thaliana
4.1.1.49 citrate
-
 Arabidopsis thaliana
4.1.1.49 D-fructose 1,6-bisphosphate
-
 Arabidopsis thaliana
4.1.1.49 D-fructose 6-phosphate
-
 Arabidopsis thaliana
4.1.1.49 D-glucose 1-phosphate
-
 Arabidopsis thaliana
4.1.1.49 D-glucose 6-phosphate
-
 Arabidopsis thaliana
4.1.1.49 diphosphate
-
 Arabidopsis thaliana
4.1.1.49 fumarate
-
 Arabidopsis thaliana
4.1.1.49 L-glutamate
-
 Arabidopsis thaliana
4.1.1.49 N-phosphomethylglycine
-
 Arabidopsis thaliana
4.1.1.49 pyruvate
-
 Arabidopsis thaliana
4.1.1.49 shikimate
-
 Arabidopsis thaliana
4.1.1.49 succinate
-
 Arabidopsis thaliana
4.1.1.49 UDP
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.49 0.018
-
 ATP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 0.039
-
 ADP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 0.072
-
 ATP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 0.079
-
 ADP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 0.1
-
 oxaloacetate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 0.23
-
 oxaloacetate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 3.7
-
 phosphoenolpyruvate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 3.8
-
 phosphoenolpyruvate pH 7, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.49 Mn2+ required. Highest activity is obtained at 1 mM Mn2+ in the absence of Mg2+, the presence of 0.5 mM Mg2+ decreases the activity by a factor 2 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.49 440000
-
 gel filtraion Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.49 Arabidopsis thaliana Q9T074
-
-
4.1.1.49 Arabidopsis thaliana B5X574
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.49 3
-
 pH 7, 30°C Arabidopsis thaliana
4.1.1.49 6
-
 pH 7, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.49 ADP + phosphoenolpyruvate + CO2
-
 Arabidopsis thaliana ATP + oxaloacetate
-
 r
4.1.1.49 ATP + oxaloacetate
-
 Arabidopsis thaliana ADP + phosphoenolpyruvate + CO2
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.1.49 hexamer 6 * 73500, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.1.1.49 At4g37870
-
 Arabidopsis thaliana
4.1.1.49 At5g65690
-
 Arabidopsis thaliana
4.1.1.49 PCK1
-
 Arabidopsis thaliana
4.1.1.49 PCK2
-
 Arabidopsis thaliana
4.1.1.49 PEPCK1
-
 Arabidopsis thaliana
4.1.1.49 PEPCK2
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.49 7
-
 both carboxylation and decarboxylation Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.49 Arabidopsis thaliana calculated from sequence
-
 6.6

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.49 1 3 Hill coefficient 1.7, pH 7, 30°C Arabidopsis thaliana 2-oxoglutarate
4.1.1.49 2.1
-
 Hill coefficient 1.4, pH 7, 30°C Arabidopsis thaliana citrate
4.1.1.49 2.7
-
 Hill coefficient 1.1, pH 7, 30°C Arabidopsis thaliana diphosphate
4.1.1.49 3.7
-
 Hill coefficient 1.5, pH 7, 30°C Arabidopsis thaliana diphosphate
4.1.1.49 3.7
-
 Hill coefficient 1.4, pH 7, 30°C Arabidopsis thaliana citrate
4.1.1.49 6.1
-
 Hill coefficient 0.8, pH 7, 30°C Arabidopsis thaliana N-phosphomethylglycine
4.1.1.49 8
-
 Hill coefficient 0.8, pH 7, 30°C Arabidopsis thaliana UDP
4.1.1.49 8.1
-
 Hill coefficient 1.5, pH 7, 30°C Arabidopsis thaliana D-glucose 6-phosphate
4.1.1.49 8.6
-
 Hill coefficient 1.7, pH 7, 30°C Arabidopsis thaliana fumarate
4.1.1.49 9.6
-
 presence of Mg2+, Hill coefficient 1.9, pH 7, 30°C Arabidopsis thaliana fumarate
4.1.1.49 10
-
 Hill coefficient 1.8, pH 7, 30°C Arabidopsis thaliana fumarate
4.1.1.49 10.3
-
 presence of Mg2+, Hill coefficient 1.5, pH 7, 30°C Arabidopsis thaliana citrate
4.1.1.49 10.4
-
 Hill coefficient 1.3, pH 7, 30°C Arabidopsis thaliana shikimate
4.1.1.49 11
-
 Hill coefficient 0.9, pH 7, 30°C Arabidopsis thaliana succinate
4.1.1.49 11
-
 presence of Mg2+, Hill coefficient 2.3, pH 7, 30°C Arabidopsis thaliana citrate
4.1.1.49 12.1
-
 Hill coefficient 1.4, pH 7, 30°C Arabidopsis thaliana succinate
4.1.1.49 14.5
-
 presence of Mg2+, Hill coefficient 3.5, pH 7, 30°C Arabidopsis thaliana succinate
4.1.1.49 15
-
 Hill coefficient 1.1, pH 7, 30°C Arabidopsis thaliana pyruvate
4.1.1.49 16
-
 Hill coefficient 1.2, pH 7, 30°C Arabidopsis thaliana D-fructose 1,6-bisphosphate
4.1.1.49 18
-
 presence of Mg2+, Hill coefficient 3.7, pH 7, 30°C Arabidopsis thaliana succinate
4.1.1.49 19
-
 presence of Mg2+, Hill coefficient 1.1, pH 7, 30°C Arabidopsis thaliana 2-oxoglutarate
4.1.1.49 21
-
 Hill coefficient 1.5, pH 7, 30°C Arabidopsis thaliana 3-phosphoglycerate
4.1.1.49 21
-
 Hill coefficient 0.9, pH 7, 30°C Arabidopsis thaliana N-phosphomethylglycine
4.1.1.49 26
-
 Hill coefficient 1.5, pH 7, 30°C Arabidopsis thaliana D-glucose 1-phosphate
4.1.1.49 27
-
 Hill coefficient 0.9, pH 7, 30°C Arabidopsis thaliana D-fructose 6-phosphate
4.1.1.49 27
-
 Hill coefficient 2.9, pH 7, 30°C Arabidopsis thaliana pyruvate
4.1.1.49 32
-
 Hill coefficient 0.9, pH 7, 30°C Arabidopsis thaliana L-glutamate
4.1.1.49 35
-
 Hill coefficient 0.9, pH 7, 30°C Arabidopsis thaliana 3-phosphoglycerate
4.1.1.49 41
-
 Hill coefficient 0.4, pH 7, 30°C Arabidopsis thaliana 2-oxoglutarate
4.1.1.49 43
-
 Hill coefficient 0.8, pH 7, 30°C Arabidopsis thaliana D-fructose 1,6-bisphosphate

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.49 0.5
-
 phosphoenolpyruvate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 1.1
-
 phosphoenolpyruvate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 13
-
 oxaloacetate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 25
-
 ADP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 39
-
 ATP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 82
-
 oxaloacetate pH 7, 30°C Arabidopsis thaliana
4.1.1.49 100
-
 ADP pH 7, 30°C Arabidopsis thaliana
4.1.1.49 370
-
 ATP pH 7, 30°C Arabidopsis thaliana