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Literature summary extracted from

  • Chen, X.; Chen, J.; Zhang, W.; Wang, H.; Liu, X.; Zhou, W.; Yang, H.; Rao, Z.
    Crystal structure and biochemical study on argininosuccinate lyase from Mycobacterium tuberculosis (2019), Biochem. Biophys. Res. Commun., 510, 116-121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.1 expressed in Escherichia coli Rosetta cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.1 vapor diffusion method, apoenzyme: 8% (v/v) tacsimate, 28% (w/v) polyethylene glycol 3350. Enzyme bound to SO4: 0.2 M ammonium tartrate dibasic, pH 7.0, and 20% (w/v) PEG 3350 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.1 H161A inactive Mycobacterium tuberculosis
4.3.2.1 K159A the mutant is nearly inactive Mycobacterium tuberculosis
4.3.2.1 K159Y the activity of mutant K159Y is higher than that of K159A, but lower than the wild type Mycobacterium tuberculosis
4.3.2.1 N115A inactive Mycobacterium tuberculosis
4.3.2.1 S164R the mutant is nearly inactive Mycobacterium tuberculosis
4.3.2.1 S282A inactive Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.1 1.55
-
 2-(Nomega-L-arginino)succinate wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.1 2-(Nomega-L-arginino)succinate Mycobacterium tuberculosis
-
 fumarate + L-arginine
-
 r
4.3.2.1 2-(Nomega-L-arginino)succinate Mycobacterium tuberculosis H37Rv
-
 fumarate + L-arginine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.1 Mycobacterium tuberculosis P9WPY7
-
-
4.3.2.1 Mycobacterium tuberculosis H37Rv P9WPY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.1 Ni-NTA column chromatography HiTrap Q column chromatography, and Superdex G200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.1 2-(Nomega-L-arginino)succinate
-
 Mycobacterium tuberculosis fumarate + L-arginine
-
 r
4.3.2.1 2-(Nomega-L-arginino)succinate
-
 Mycobacterium tuberculosis H37Rv fumarate + L-arginine
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.2.1 homotetramer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.3.2.1 ASL
-
 Mycobacterium tuberculosis
4.3.2.1 Rv1659
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.1 9.39
-
 2-(Nomega-L-arginino)succinate wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
4.3.2.1 physiological function the enzyme is essential for growth of Mycobacterium tuberculosis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.2.1 6.06
-
 2-(Nomega-L-arginino)succinate wild type enzyme, at pH 7.5 and 25°C Mycobacterium tuberculosis