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Literature summary extracted from

  • Lemke, C.; Roach, K.; Ortega, T.; Tantillo, D.J.; Siegel, J.B.; Peters, R.J.
    Investigation of acid-base catalysis in halimadienyl diphosphate synthase involved in Mycobacterium tuberculosis virulence (2022), ACS Bio Med Chem Au, 2, 490-498.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.16 expressed in Escherichia coli BL21-Star cells Mycobacterium tuberculosis
5.5.1.30 expressed in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.16 A234N/F235V the mutant predominantly produces tuberculosinyl diphosphate, with trace amounts of labda-8,13-dienyl diphosphate and labda-7,13-dienyl diphosphate from geranylgeranyl diphosphate Mycobacterium tuberculosis
5.5.1.16 D295A almost completely inactive Mycobacterium tuberculosis
5.5.1.16 F171A the mutant forms small amounts of tuberculosinyl diphosphate and labda-8,13-dienyl diphosphate, along with trace amounts of labda-7,13-dienyl diphosphate from geranylgeranyl diphosphate Mycobacterium tuberculosis
5.5.1.16 F171H the mutant generates significant amounts of copalyl diphosphate from geranylgeranyl diphosphate Mycobacterium tuberculosis
5.5.1.16 F171H/A234N/F235V the mutations lead to an almost complete loss of metabolic flux Mycobacterium tuberculosis
5.5.1.16 Y328F the mutant generates an alternative product (labda-7,13-dienyl diphosphate) from geranylgeranyl diphosphate Mycobacterium tuberculosis
5.5.1.16 Y328F/Y479F the mutant generates an alternative product (labda-7,13-dienyl diphosphate) from geranylgeranyl diphosphate Mycobacterium tuberculosis
5.5.1.16 Y479F the mutant generates an alternative product (labda-7,13-dienyl diphosphate) from geranylgeranyl diphosphate Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.16 geranylgeranyl diphosphate Mycobacterium tuberculosis
-
 tuberculosinyl diphosphate
-
 ?
5.5.1.16 geranylgeranyl diphosphate Mycobacterium tuberculosis H37Rv
-
 tuberculosinyl diphosphate
-
 ?
5.5.1.30 geranylgeranyl diphosphate Mycobacterium tuberculosis the enzyme produces small amounts of halima-5,13-dienyl diphosphate and labda-8,13-dienyl diphosphate, along with trace amounts of labda-7,13-dienyl diphosphate, as well as an unknown product halima-5,13-dienyl diphosphate + labda-8,13-dienyl diphosphate + labda-7,13-dienyl diphosphate + ?
-
 ?
5.5.1.30 geranylgeranyl diphosphate Mycobacterium tuberculosis H37Rv the enzyme produces small amounts of halima-5,13-dienyl diphosphate and labda-8,13-dienyl diphosphate, along with trace amounts of labda-7,13-dienyl diphosphate, as well as an unknown product halima-5,13-dienyl diphosphate + labda-8,13-dienyl diphosphate + labda-7,13-dienyl diphosphate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.16 Mycobacterium tuberculosis O50406
-
-
5.5.1.16 Mycobacterium tuberculosis H37Rv O50406
-
-
5.5.1.30 Mycobacterium tuberculosis
-
-
-
5.5.1.30 Mycobacterium tuberculosis H37Rv
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.16 geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis tuberculosinyl diphosphate
-
 ?
5.5.1.16 geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis H37Rv tuberculosinyl diphosphate
-
 ?
5.5.1.30 geranylgeranyl diphosphate the enzyme produces small amounts of halima-5,13-dienyl diphosphate and labda-8,13-dienyl diphosphate, along with trace amounts of labda-7,13-dienyl diphosphate, as well as an unknown product Mycobacterium tuberculosis halima-5,13-dienyl diphosphate + labda-8,13-dienyl diphosphate + labda-7,13-dienyl diphosphate + ?
-
 ?
5.5.1.30 geranylgeranyl diphosphate the enzyme produces small amounts of halima-5,13-dienyl diphosphate and labda-8,13-dienyl diphosphate, along with trace amounts of labda-7,13-dienyl diphosphate, as well as an unknown product Mycobacterium tuberculosis H37Rv halima-5,13-dienyl diphosphate + labda-8,13-dienyl diphosphate + labda-7,13-dienyl diphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.16 halimadienyl diphosphate synthase
-
 Mycobacterium tuberculosis
5.5.1.16 HPS
-
 Mycobacterium tuberculosis
5.5.1.16 Rv3377c
-
 Mycobacterium tuberculosis
5.5.1.30 HPS:F171A mutant F171A of class II diterpene cyclase Rv3377c Mycobacterium tuberculosis