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Literature summary extracted from

  • Gibu, N.; Kasai, D.; Ikawa, T.; Akiyama, E.; Fukuda, M.
    Characterization and transcriptional regulation of n-alkane hydroxylase gene cluster of Rhodococcus jostii RHA1 (2019), Microorganisms, 7, 479.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.3 gene alkB, DNA and amino acid sequence determination and analysis, genetic organisations, quantitative reverse transcription- and real-time PCR enzyme expression analysis Rhodococcus jostii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.3 additional information alkB and alkU genes are separately disrupted using the sacB counterselection system, and are confirmed by diagnostic PCR using specific primer sets and subsequently by DNA sequencing of the PCR amplified regions flanking the deletions. When the alkB mutation is complemented by the introduction of the corresponding gene in trans, the growth deficiency is restored. When mutant DAB is grown in n-dodecane and n-decane, the growths decreased Rhodococcus jostii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 additional information Rhodococcus jostii in vivo substrate specificity versus n-alkanes by strain RHA1, overview ?
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ Rhodococcus jostii low activity 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-undecane + 2 reduced rubredoxin + O2 + 2 H+ Rhodococcus jostii
-
 1-undecanol + 2 oxidized rubredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Rhodococcus jostii Q0SDP7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.3 cell culture strain RHA1 grows on n-alkanes of chain lengths C11 to C19 in liquid media and of chain lengths C10-C13 in solid media, overview Rhodococcus jostii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 additional information in vivo substrate specificity versus n-alkanes by strain RHA1, overview Rhodococcus jostii ?
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ low activity Rhodococcus jostii 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+
-
 Rhodococcus jostii 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-undecane + 2 reduced rubredoxin + O2 + 2 H+
-
 Rhodococcus jostii 1-undecanol + 2 oxidized rubredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.3 AlkB
-
 Rhodococcus jostii
1.14.15.3 n-alkane 1-monooxygena
-
 Rhodococcus jostii
1.14.15.3 n-alkane hydroxylase
-
 Rhodococcus jostii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 rubredoxin
-
 Rhodococcus jostii

Expression

EC Number Organism Comment Expression
1.14.15.3 Rhodococcus jostii alkB is involved in the medium-chain n-alkanes degradation of strain RHA1 and the transcription of alk operon is negatively regulated by alkU-encoded regulator. AlkU is able to bind to the putative promoter region of alkB. N-Alkane degradation pathway of Rhodococcus jostii, including the transcriptional regulation of the alk gene cluster down

General Information

EC Number General Information Comment Organism
1.14.15.3 metabolism transcriptional regulation of alkB involving alkU, mutational analysis, overview Rhodococcus jostii
1.14.15.3 physiological function gene alkB is not essential for the utilization of n-hexadecane, other gene(s) are involved in this degradation Rhodococcus jostii