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Literature summary extracted from

  • Li, T.; Gao, Y.Z.; Xu, J.; Zhang, S.T.; Guo, Y.; Spain, J.C.; Zhou, N.Y.
    A Recently assembled degradation pathway for 2,3-dichloronitrobenzene in Diaphorobacter sp. Strain JS3051 (2021), mBio, 12, e0223121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.23 expressed in Escherichia coli BL21(DE3) cells Diaphorobacter sp. JS3051

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.23 E204I the mutation causes a shift in preference from meta- to ortho-substituted substrates, such as 2-chloronitrobenzene, 2-nitrotoluene, and 2,3-dichloronitrobenzene. Unexpectedly, 2,3-dichloronitrobenzene is still a primary substrate of the variant Diaphorobacter sp. JS3051

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.23 Diaphorobacter sp. JS3051
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.23 2,3-dichloronitrobenzene + NADH + O2 highest activity Diaphorobacter sp. JS3051 3,4-dichlorocatechol + nitrite + NAD+
-
 ?
1.14.12.23 2-dichloronitrobenzene + NADH + O2 low activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
1.14.12.23 2-nitrotoluene + NADH + O2 low activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
1.14.12.23 3,4-dichloronitrobenzene + NADH + O2 low activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
1.14.12.23 3-chloronitrobenzene + NADH + O2 second highest activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
1.14.12.23 3-nitrotoluene + NADH + O2 third highest activity Diaphorobacter sp. JS3051 ? + nitrite + NAD+
-
 ?
1.14.12.23 additional information no activity with 2,4-dichloronitrobenzene, 4-nitrotoluene, 4-dichloronitrobenzene, and nitrobenzene Diaphorobacter sp. JS3051 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.23 2,3-dichloronitrobenzene dioxygenase
-
 Diaphorobacter sp. JS3051
1.14.12.23 23DCNB dioxygenase
-
 Diaphorobacter sp. JS3051
1.14.12.23 23DCNBDO
-
 Diaphorobacter sp. JS3051
1.14.12.23 DcbAaAbAcAd
-
 Diaphorobacter sp. JS3051

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.23 NADH
-
 Diaphorobacter sp. JS3051