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Literature summary extracted from

  • Uchida, H.; Sumimoto, K.; Oki, T.; Nishii, I.; Mizohata, E.; Matsunaga, S.; Okada, S.
    Isolation and characterization of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase gene from Botryococcus braunii, race B (2018), J. Plant Res., 131, 839-848.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.17.7.4 biofuel production the B race of a green microalga Botryococcus braunii Kützing produces triterpene hydrocarbons that is a promising source for biofuel. In this algal race, precursors of triterpene hydrocarbons are provided from the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway Botryococcus braunii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.4 gene HDR, single copy gene, cloning from cDNA and genomic DNA, DNA and amino acid sequence determination and analysis, genetic structure, quantitative RT-PCR enzyme expression analysis. Phylogenetic analysis revealing that exon-intron structure of BbHDR genes does not show clear relation to phylogeny, while its amino acid sequence reflects phyla and classes well, phylogenetic tree. Introduction of BbHDR cDNA into an Escherichia coli HDR-deficient mutant results in recovery of its auxotrophy, recombinant enzyme expression in HDR-null Escherichia coli strain MG1655 Botryococcus braunii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.4 Fe2+ required Botryococcus braunii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Botryococcus braunii
-
 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Botryococcus braunii Showa
-
 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Botryococcus braunii
-
 prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Botryococcus braunii Showa
-
 prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.4 Botryococcus braunii A0A2Z5WA18 B race
-
1.17.7.4 Botryococcus braunii Showa A0A2Z5WA18 B race
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.7.4 additional information gene expression analysis during a culture period, overview Botryococcus braunii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Botryococcus braunii 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Botryococcus braunii Showa 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Botryococcus braunii prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Botryococcus braunii Showa prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.7.4 BbHDR
-
 Botryococcus braunii
1.17.7.4 HDR
-
 Botryococcus braunii

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.7.4 Ferredoxin
-
 Botryococcus braunii

Expression

EC Number Organism Comment Expression
1.17.7.4 Botryococcus braunii hydrocarbon content is positively correlated with BbHDR expression. Upregulation of BbHDR expression remains within a smaller amplitude up

General Information

EC Number General Information Comment Organism
1.17.7.4 metabolism in the B race of the algal species Botryococcus braunii, precursors of triterpene hydrocarbons are provided from the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. The terminal enzyme of this pathway, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR), is regarded as one of the key enzymes that affect yields of products in terpene biosynthesis Botryococcus braunii
1.17.7.4 additional information three-dimensional structure modeling using a crystal structure of HDR from Escherichia coli (PDB ID 3F7T) as a template, overview Botryococcus braunii