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Literature summary extracted from

  • Stopamo, F.; Rohr, A.; Mekasha, S.; Petrovi, D.; Varnai, A.; Eijsink, V.
    Characterization of a lytic polysaccharide monooxygenase from Aspergillus fumigatus shows functional variation among family AA11 fungal LPMOs (2021), J. Biol. Chem., 297, 101421.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.53 H2O2 catalytic activity of AA11A on chitin increases in presence of H2O2 Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53 expression in Pichia pastoris Aspergillus fumigatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.53 structure shows a substrate-binding surface with features similar to known chitin-active LPMOs and the absence of a carbohydrate-binding module Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Aspergillus fumigatus Q4WF00
-
-
1.14.99.53 Aspergillus fumigatus ATCC MYA-4609 Q4WF00
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.99.53 glycoprotein sequence contains a possible N-glycosylation site, at Asn62. Treatment with endo-beta-N-acetylglucosaminidase leads to a mass reduction to 22 kDA Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.53 recombinant protein Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.53 alpha-chitin + gallate + O2
-
 Aspergillus fumigatus chito-oligosaccharide C1-aldonic acids + dehydroascorbate + H2O
-
 ?
1.14.99.53 alpha-chitin + gallate + O2
-
 Aspergillus fumigatus ATCC MYA-4609 chito-oligosaccharide C1-aldonic acids + dehydroascorbate + H2O
-
 ?
1.14.99.53 beta-chitin + ascorbate + O2
-
 Aspergillus fumigatus ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 beta-chitin + ascorbate + O2
-
 Aspergillus fumigatus ATCC MYA-4609 ? + dehydroascorbate + H2O
-
 ?
1.14.99.53 additional information presence of ascorbate is required. No substrates: phosphoric acid-swollen cellulose, avicel, tamarind xyloglucan, birchwood xylan, beechwood xylan, acetyl glucuronoxylan from aspen, ivory nut mannan, acetylated konjac glucomannan, potato starch, heparin, hyaluronic acid, and chitosan Aspergillus fumigatus ?
-
 ?
1.14.99.53 additional information presence of ascorbate is required. No substrates: phosphoric acid-swollen cellulose, avicel, tamarind xyloglucan, birchwood xylan, beechwood xylan, acetyl glucuronoxylan from aspen, ivory nut mannan, acetylated konjac glucomannan, potato starch, heparin, hyaluronic acid, and chitosan Aspergillus fumigatus ATCC MYA-4609 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.53 ? x * 30000, SDS-PAGE, x * 21750, calculated from sequence Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.53 30
-
 assay at Aspergillus fumigatus