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Literature summary extracted from

  • Kadri, T.; Rouissi, T.; Magdouli, S.; Brar, S.K.; Hegde, K.; Khiari, Z.; Daghrir, R.; Lauzon, J.M.
    Production and characterization of novel hydrocarbon degrading enzymes from Alcanivorax borkumensis (2018), Int. J. Biol. Macromol., 112, 230-240.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Alcanivorax borkumensis Q0VKZ3
-
-
1.14.15.3 Alcanivorax borkumensis Q0VTH3
-
-
1.14.15.3 Alcanivorax borkumensis ATCC 700651 Q0VKZ3
-
-
1.14.15.3 Alcanivorax borkumensis ATCC 700651 Q0VTH3
-
-
1.14.15.3 Alcanivorax borkumensis DSM 11573 Q0VKZ3
-
-
1.14.15.3 Alcanivorax borkumensis DSM 11573 Q0VTH3
-
-
1.14.15.3 Alcanivorax borkumensis NCIMB 13689 Q0VKZ3
-
-
1.14.15.3 Alcanivorax borkumensis NCIMB 13689 Q0VTH3
-
-
1.14.15.3 Alcanivorax borkumensis SK2 Q0VKZ3
-
-
1.14.15.3 Alcanivorax borkumensis SK2 Q0VTH3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 additional information remediation by the enzyme of petroleum hydrocarbons, hexane, hexadecane and motor oil as model substrates. Hexane can be degraded by the alkane hydroxylase AlkB1, hexadecane can be degraded by the alkane hydroxylase AlkB2 and the motor oil can be degraded by both AlkB1 and AlkB2, since it contains a mixture of C10-C50 other than monoaromatic and polyaromatic hydrocarbons Alcanivorax borkumensis ?
-
 ?
1.14.15.3 additional information remediation by the enzyme of petroleum hydrocarbons, hexane, hexadecane and motor oil as model substrates. Hexane can be degraded by the alkane hydroxylase AlkB1, hexadecane can be degraded by the alkane hydroxylase AlkB2 and the motor oil can be degraded by both AlkB1 and AlkB2, since it contains a mixture of C10-C50 other than monoaromatic and polyaromatic hydrocarbons Alcanivorax borkumensis SK2 ?
-
 ?
1.14.15.3 additional information remediation by the enzyme of petroleum hydrocarbons, hexane, hexadecane and motor oil as model substrates. Hexane can be degraded by the alkane hydroxylase AlkB1, hexadecane can be degraded by the alkane hydroxylase AlkB2 and the motor oil can be degraded by both AlkB1 and AlkB2, since it contains a mixture of C10-C50 other than monoaromatic and polyaromatic hydrocarbons Alcanivorax borkumensis ATCC 700651 ?
-
 ?
1.14.15.3 additional information remediation by the enzyme of petroleum hydrocarbons, hexane, hexadecane and motor oil as model substrates. Hexane can be degraded by the alkane hydroxylase AlkB1, hexadecane can be degraded by the alkane hydroxylase AlkB2 and the motor oil can be degraded by both AlkB1 and AlkB2, since it contains a mixture of C10-C50 other than monoaromatic and polyaromatic hydrocarbons Alcanivorax borkumensis DSM 11573 ?
-
 ?
1.14.15.3 additional information remediation by the enzyme of petroleum hydrocarbons, hexane, hexadecane and motor oil as model substrates. Hexane can be degraded by the alkane hydroxylase AlkB1, hexadecane can be degraded by the alkane hydroxylase AlkB2 and the motor oil can be degraded by both AlkB1 and AlkB2, since it contains a mixture of C10-C50 other than monoaromatic and polyaromatic hydrocarbons Alcanivorax borkumensis NCIMB 13689 ?
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 86.6% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 86.6% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis SK2 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 86.6% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis ATCC 700651 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 86.6% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis DSM 11573 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 86.6% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis NCIMB 13689 1-hexadecanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 56% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis 1-hexanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 56% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis SK2 1-hexanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 56% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis ATCC 700651 1-hexanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 56% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis DSM 11573 1-hexanol + 2 oxidized rubredoxin + H2O
-
 ?
1.14.15.3 n-hexane + 2 reduced rubredoxin + O2 + 2 H+ during 10 days of incubation, 56% of HXD is degraded by strain RFC-1 at 75°C Alcanivorax borkumensis NCIMB 13689 1-hexanol + 2 oxidized rubredoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.3 alkane 1-monooxygenase 1 UniProt Alcanivorax borkumensis
1.14.15.3 alkane 1-monooxygenase 2 UniProt Alcanivorax borkumensis
1.14.15.3 alkane hydroxylase
-
 Alcanivorax borkumensis
1.14.15.3 AlkB1
-
 Alcanivorax borkumensis
1.14.15.3 AlkB2
-
 Alcanivorax borkumensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.3 70
-
 with substrate motor oil, crude enzyme extract Alcanivorax borkumensis
1.14.15.3 75
-
 with substrates hexane and hexadecane, crude enzyme extract Alcanivorax borkumensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.15.3 50 85 crude enzyme extract, with hexadecane and hexane: optimum activity at 70°C, 40% of maximal activity at 60°C and 80°C, poor activity below 50°C and no activity above 90°C. With motor oil: optimum activity at 75°C, 60% of maximal activity at 65°C and 85°C, poor activity below 40°C and no activity above 90-95°C. Profiles overview Alcanivorax borkumensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.3 8
-
 with all substrates, crude enzyme extract Alcanivorax borkumensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.15.3 3 10 activity range with substrates hexane and hexadecane and with motor oil, crude enzyme extract, profiles overview Alcanivorax borkumensis

General Information

EC Number General Information Comment Organism
1.14.15.3 physiological function Alcanivorax borkumensis strain SK2 possesses an AlkB1 alkane hydroxylase that can oxidize medium chain alkanes in the range C5 to C12 and an alkane hydroxylase AlkB2, that oxidizes medium-chain alkanes in the range C8 to C16. It has also been claimed that Alcanivorax borkumensis strain SK2 is able to degrade a large range of alkanes up to C32 and branched aliphatic, as well as isoprenoid hydrocarbons (e.g. phytane), alkylarenes and alkylcycloalkanes. Consequently, hexane can be degraded by the alkane hydroxylase AlkB1, hexadecane can be degraded by the alkane hydroxylase AlkB2 and the motor oil can be degraded by both AlkB1 and AlkB2, since it contains a mixture of C10-C50 other than monoaromatic and polyaromatic hydrocarbons. The additional existence of different lipases/esterases allows the strain to grow on hexane, hexadecane, and motor oil at high concentrations Alcanivorax borkumensis