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Literature summary extracted from

  • Sagi-Kazar, M.; Zelenyanszki, H.; Mueller, B.; Cseh, B.; Gyuris, B.; Farkas, S.Z.; Fodor, F.; Toth, B.; Kovacs, B.; Koncz, A.; Visnovitz, T.; Buzas, E.I.; Bankuti, B.; Banati, F.; Szenthe, K.; Solti, A.
    Supraoptimal iron nutrition of Brassica napus plants suppresses the iron uptake of chloroplasts by down-regulating chloroplast ferric chelate reductase (2021), Front. Plant Sci., 12, 658987.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.1.9 expressed in Phaseolus vulgaris leaf chloroplasts Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.1.9 0.02207
-
Fe(III)-EDTA at optimal iron nutrition, pH and temperature not specified in the publication Brassica napus
1.16.1.9 0.02346
-
Fe(III)-EDTA at iron deficiency, pH and temperature not specified in the publication Brassica napus
1.16.1.9 0.03022
-
Fe(III)-citrate at suboptimal iron nutrition, pH and temperature not specified in the publication Brassica napus
1.16.1.9 0.04637
-
Fe(III)-citrate at iron deficiency, pH and temperature not specified in the publication Brassica napus
1.16.1.9 0.047
-
Fe(III)-citrate at optimal iron nutrition, pH and temperature not specified in the publication Brassica napus
1.16.1.9 0.05252
-
Fe(III)-EDTA at suboptimal iron nutrition, pH and temperature not specified in the publication Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.1.9 chloroplast inner chloroplast envelope membrane Brassica napus 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.16.1.9 Mg2+ 2 mM used in assay conditions Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.9 Brassica napus
-
cultivar DK Exquisite
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.16.1.9 leaf
-
Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.9 2 Fe(III)-citrate + NADPH
-
Brassica napus 2 Fe(II)-citrate + NADP+ + H+
-
?
1.16.1.9 2 Fe(III)-EDTA + NADPH
-
Brassica napus 2 Fe(II)-EDTA + NADP+ + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.16.1.9 FCR
-
Brassica napus
1.16.1.9 ferric reductase oxidase 7
-
Brassica napus
1.16.1.9 FRO7
-
Brassica napus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.16.1.9 57.5
-
melting temperature Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.9 FAD
-
Brassica napus
1.16.1.9 NADPH
-
Brassica napus

Expression

EC Number Organism Comment Expression
1.16.1.9 Brassica napus iron deficiency does not increase isoform FRO7 expression additional information