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Literature summary extracted from

  • Benes, V.; Leonhardt, T.; Sacky, J.; Kotrba, P.
    Two P1B-1-ATPases of Amanita strobiliformis with distinct properties in Cu/Ag transport (2018), Front. Microbiol., 9, 747.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.8 expression in Saccharomyces cerevisiae Amanita strobiliformis
7.2.2.15 expression in Saccharomyces cerevisiae Amanita strobiliformis

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.8 D555A AsCCC2D555A cells show full growth on the YPEG medium supplemented with 1 mM Cu2+ Amanita strobiliformis
7.2.2.15 D555A AsCCC2D555A cells show full growth on the YPEG medium supplemented with 1 mM Cu2+ Amanita strobiliformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.8 ATP + H2O + Ag+[side 1] Amanita strobiliformis
-
 ADP + phosphate + Ag+[side 2]
-
 ?
7.2.2.15 ATP + H2O + Ag+[side 1] Amanita strobiliformis
-
 ADP + phosphate + Ag+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.8 Amanita strobiliformis A0A2S1JL61 cf. EC 7.2.2.15
-
7.2.2.8 Amanita strobiliformis A0A2S1JL48 cf. EC 7.2.2.15
-
7.2.2.15 Amanita strobiliformis A0A2S1JL61 cf. EC 7.2.2.8
-
7.2.2.15 Amanita strobiliformis A0A2S1JL48 cf. EC 7.2.2.8
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.8 ATP + H2O + Ag+[side 1]
-
 Amanita strobiliformis ADP + phosphate + Ag+[side 2]
-
 ?
7.2.2.8 ATP + H2O + Cu+[side 1]
-
 Amanita strobiliformis ADP + phosphate + Cu+[side 2]
-
 ?
7.2.2.15 ATP + H2O + Ag+[side 1]
-
 Amanita strobiliformis ADP + phosphate + Ag+[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.8 AsCCC2
-
 Amanita strobiliformis
7.2.2.8 AsCRD1
-
 Amanita strobiliformis
7.2.2.8 P1B-1-ATPase
-
 Amanita strobiliformis
7.2.2.15 AsCCC2
-
 Amanita strobiliformis
7.2.2.15 AsCRD1
-
 Amanita strobiliformis
7.2.2.15 P1B-1-ATPase
-
 Amanita strobiliformis

Expression

EC Number Organism Comment Expression
7.2.2.15 Amanita strobiliformis treatments of mycelia with Ag+ and Cu+ elevates the expression of AsCRD1 relative to the unexposed control. The average levels of AsCRD1 transcripts increase 4.5- and 8.7-fold in the presence of 5 mM Ag+ and Cu2+, respectively, and they further nearly double when the concentration of the two metals is 50 mM up

General Information

EC Number General Information Comment Organism
7.2.2.8 physiological function the enzyme supports the detoxification of Ag and Cu, which is, besides efficient transport, the prerequisite for (hyper)accumulation Amanita strobiliformis
7.2.2.8 physiological function the enzyme is involved in the delivery of physiological Cu into organelles of endomembrane system for the biosynthesis of Cu-dependent proteins Amanita strobiliformis
7.2.2.15 physiological function the enzyme supports the detoxification of Ag and Cu, which is, besides efficient transport, the prerequisite for (hyper)accumulation Amanita strobiliformis
7.2.2.15 physiological function the enzyme is involved in the delivery of physiological Cu into organelles of endomembrane system for the biosynthesis of Cu-dependent proteins Amanita strobiliformis