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Literature summary extracted from

  • Kurauchi, T.; Matsui, K.; Shimasaki, T.; Ohtsuka, H.; Tsubouchi, S.; Ihara, K.; Tani, M.; Aiba, H.
    Identification of sur2 mutation affecting the lifespan of fission yeast (2021), FEMS Microbiol. Lett., 368, 0000.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.5 gene sur2, genotyping, recombinant expression of GST-tagged enzyme in enzyme-deficient Schizosaccharomyces pombe strain JY333 Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
1.14.18.5 additional information the naturally occuring sur2-L1 mutation in the L1 mutant causes a long-lived phenotype, the sur2-L1 mutation is almost a loss-of-function mutation. Construction of a sur2-L1::kanR mutant. The wild-type and sur2DELTA mutant of Saccharomyces cerevisiae are used as controls for the composition of sphingolipids, comparisons of composition of sphingolipids in wild-type and mutant cells Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.5 dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ Schizosaccharomyces pombe
-
 (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O
-
 ?
1.14.18.5 dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ Schizosaccharomyces pombe 972
-
 (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O
-
 ?
1.14.18.5 dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ Schizosaccharomyces pombe ATCC 24843
-
 (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.5 Schizosaccharomyces pombe O94298
-
-
1.14.18.5 Schizosaccharomyces pombe 972 O94298
-
-
1.14.18.5 Schizosaccharomyces pombe ATCC 24843 O94298
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.5 dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Schizosaccharomyces pombe (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O
-
 ?
1.14.18.5 dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Schizosaccharomyces pombe 972 (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O
-
 ?
1.14.18.5 dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Schizosaccharomyces pombe ATCC 24843 (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.5 sphingolipid C4-hydroxylase
-
 Schizosaccharomyces pombe
1.14.18.5 SUR2
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.5 30
-
 assay at Schizosaccharomyces pombe

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.5 cytochrome b5
-
 Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
1.14.18.5 malfunction the missense mutation in the sur2+ gene, which encodes a homolog of Saccharomyces cerevisiae sphingolipid C4-hydroxylase in fission yeast, causes the loss of sur2 function which results in an extended chronological lifespan. The effect of caloric restriction, a well-known signal for extending lifespan, is thought to be dependent on the sur2+ gene. The sur2-L1 mutation is almost a loss-of-function mutation Schizosaccharomyces pombe
1.14.18.5 additional information comparisons of composition of sphingolipids in wild-type and mutant cells Schizosaccharomyces pombe
1.14.18.5 physiological function normal amount of Sur2 protein is required to maintain a normal lifespan, and that imbalance due to decreased or increased Sur2 activity may extend lifespan Schizosaccharomyces pombe