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Literature summary extracted from
- Newly isolated alkane hydroxylase and lipase producing geobacillus and anoxybacillus species involved in crude oil degradation (2020), Catalysts, 10, 851.
No PubMed abstract available
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | Geobacillus sp. D4 | - |
1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? |  |
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | Geobacillus sp. D7 | - |
1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | Anoxybacteroides rupiense | - |
1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | Anoxybacteroides rupiense D9 | - |
1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.3 | Anoxybacteroides rupiense | - |
- |
- |
| 1.14.15.3 | Anoxybacteroides rupiense D9 | - |
- |
- |
| 1.14.15.3 | Geobacillus sp. D4 | - |
- |
- |
| 1.14.15.3 | Geobacillus sp. D7 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | - |
Geobacillus sp. D4 | 1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | - |
Geobacillus sp. D7 | 1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | - |
Anoxybacteroides rupiense | 1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
| 1.14.15.3 | n-hexadecane + 2 reduced rubredoxin + O2 + 2 H+ | - |
Anoxybacteroides rupiense D9 | 1-hexadecanol + 2 oxidized rubredoxin + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.15.3 | alkane hydroxylase | - |
Geobacillus sp. D4 |
| 1.14.15.3 | alkane hydroxylase | - |
Geobacillus sp. D7 |
| 1.14.15.3 | alkane hydroxylase | - |
Anoxybacteroides rupiense |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.3 | 50 | - |
assay at | Anoxybacteroides rupiense |
| 1.14.15.3 | 50 | - |
assay at | Geobacillus sp. D4 |
| 1.14.15.3 | 50 | - |
assay at | Geobacillus sp. D7 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.3 | 8 | - |
assay at | Geobacillus sp. D4 |
| 1.14.15.3 | 8 | - |
assay at | Geobacillus sp. D7 |
| 1.14.15.3 | 8 | - |
assay at | Anoxybacteroides rupiense |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.3 | additional information | reaction is initiated by NADH | Geobacillus sp. D4 | |
| 1.14.15.3 | additional information | reaction is initiated by NADH | Geobacillus sp. D7 | |
| 1.14.15.3 | additional information | reaction is initiated by NADH | Anoxybacteroides rupiense | |
| 1.14.15.3 | rubredoxin | - |
Geobacillus sp. D4 | |
| 1.14.15.3 | rubredoxin | - |
Geobacillus sp. D7 | |
| 1.14.15.3 | rubredoxin | - |
Anoxybacteroides rupiense |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.15.3 | evolution | screening of thermophilic alkane hydroxylase producers is done on the east coast and the central region of peninsular Malaysia. The best three alkane hydroxylase producers are Geobacillus sp. strain D4, Geobacillus sp. strain D7, and Anoxybacillus geothermalis strain D9, morphological and biochemical identification, overview | Geobacillus sp. D4 |
| 1.14.15.3 | evolution | screening of thermophilic alkane hydroxylase producers is done on the east coast and the central region of peninsular Malaysia. The best three alkane hydroxylase producers are Geobacillus sp. strain D4, Geobacillus sp. strain D7, and Anoxybacillus geothermalis strain D9, morphological and biochemical identification, overview | Geobacillus sp. D7 |
| 1.14.15.3 | evolution | screening of thermophilic alkane hydroxylase producers is done on the east coast and the central region of peninsular Malaysia. The best three alkane hydroxylase producers are Geobacillus sp. strain D4, Geobacillus sp. strain D7, and Anoxybacillus geothermalis strain D9, morphological and biochemical identification, overview | Anoxybacteroides rupiense |
| 1.14.15.3 | physiological function | Geobacillus sp. strain D4 is an alkane degrader with 12.1% biodegradation efficiency (BE%). It is involved in crude oil biodegradation. GC analysis shows n-alkane as the major component of crude oil with 87.2% followed by 6.3% of branched alkane, 1.8% of aromatic compounds, 1.3 % of alkene, and 3.4% of trace compounds which include cyclo-alkane, resins, organosulfur, ester, organochlorine, carboxylic acids, organosilicon, and alcohol. The strain D4 is able to remove n-decane (C10) completely while a moderate breakdown of n-undecane (C11) is observed with 40.8% for D4. Degradation of n-tricosane (C23) and n-hexacosane (C26) is recorded to be more than 14% by the isolate. The isolate prefers to degrade shorter to medium-chain alkane where the degradation percentage for C15 to C17 is observed between 10.9% and 21.6%. In addition, C18 and above shows less than 10% of degradation. The isolate is able to oxidize branched alkane molecules with degradation of 3-ethylhexane (C8) and 46% to 80% degradation of 2,6,10-trimethyldodecane (C15) and 2-methyl-6-propyldodecane (C18). Longer branched alkane from C18 to C20 is being degraded at a slower rate with an average degradation percentage of 19%. A small portion of saturated cyclo-alkane molecules such as 1-nonylcycloheptane (C16) is also degraded with 21% for D4. Detailed overview over the degradation activity of strain D4 | Geobacillus sp. D4 |
| 1.14.15.3 | physiological function | Geobacillus sp. strain D7 is an alkane degrader with 17.3% biodegradation efficiency (BE%). It is involved in crude oil biodegradation. GC analysis shows n-alkane as the major component of crude oil with 87.2% followed by 6.3% of branched alkane, 1.8% of aromatic compounds, 1.3 % of alkene, and 3.4% of trace compounds which include cyclo-alkane, resins, organosulfur, ester, organochlorine, carboxylic acids, organosilicon, and alcohol. The strain D7 is able to remove n-decane (C10) completely while a moderate breakdown of n-undecane (C11) is observed with 42.4% for D7. Degradation of n-tricosane (C23) and n-hexacosane (C26) is recorded to be more than 14% by the isolate. The isolate prefers to degrade shorter to medium-chain alkane where the degradation percentage for C15 to C17 is observed between 10.9% and 21.6%. In addition, C18 and above shows less than 10% of degradation. The isolate is able to oxidize branched alkane molecules with degradation of 3-ethylhexane (C8) and 46% to 80% degradation of 2,6,10-trimethyldodecane (C15) and 2-methyl-6-propyldodecane (C18). Longer branched alkane from C18 to C20 is being degraded at a slower rate with an average degradation percentage of 19%. A small portion of saturated cyclo-alkane molecules such as 1-nonylcycloheptane (C16) is also degraded with 37.5% D7, 100% degradation of 3-ethylhexane (C8) for strain D7, which can also degrade cyclotetracosane (C24) and 1,1,3,6-tetramethyl-2-(3,6,10,13,14-pentamethyl-3-ethyl-pentadecyl) cyclohexane (C32) with an average of 13.6% removal. Detailed overview over the degradation activity of strain D7 | Geobacillus sp. D7 |
| 1.14.15.3 | physiological function | Anoxybacillus geothermalis strain D9 is an alkane degrader with 13.1% biodegradation efficiency (BE%). It is involved in crude oil biodegradation. GC analysis shows n-alkane as the major component of crude oil with 87.2% followed by 6.3% of branched alkane, 1.8% of aromatic compounds, 1.3 % of alkene, and 3.4% of trace compounds which include cyclo-alkane, resins, organosulfur, ester, organochlorine, carboxylic acids, organosilicon, and alcohol. The strain D9 is able to remove n-decane (C10) completely while a moderate breakdown of n-undecane (C11) is observed with 51% for D9. Degradation of n-tricosane (C23) and n-hexacosane (C26) is recorded to be more than 14% by the isolate. The isolate prefers to degrade shorter to medium-chain alkane where the degradation percentage for C15 to C17 is observed between 10.9% and 21.6%. In addition, C18 and above shows less than 10% of degradation. The isolate is able to oxidize branched alkane molecules with degradation of 3-ethylhexane (C8) and 46% to 80% degradation of 2,6,10-trimethyldodecane (C15) and 2-methyl-6-propyldodecane (C18). Longer branched alkane from C18 to C20 is being degraded at a slower rate with an average degradation percentage of 19%. A small portion of saturated cyclo-alkane molecules such as 1-nonylcycloheptane (C16) is also degraded with 25.2% for strain D9, which can also degrade cyclotetracosane (C24) and 1,1,3,6-tetramethyl-2-(3,6,10,13,14-pentamethyl-3-ethyl-pentadecyl) cyclohexane (C32) with an average of 13.6% removal. Detailed overview over the degradation activity of strain D9 | Anoxybacteroides rupiense |
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