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Literature summary extracted from

  • Li, D.; Wang, Q.; Chen, S.; Liu, H.; Pan, K.; Li, J.; Luo, C.; Wang, H.
    De novo assembly and analysis of Polygonatum cyrtonema Hua and identification of genes involved in polysaccharide and saponin biosynthesis (2022), BMC Genomics, 23, 195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.1 gene ispG, quantitative real-time PCR enzyme expression analysis, transcriptome analysis Polygonatum cyrtonema
1.17.7.4 gene ispH, quantitative real-time PCR enzyme expression analysis, transcriptome analysis Polygonatum cyrtonema

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.7.1 Fe2+ required Polygonatum cyrtonema
1.17.7.4 Fe2+ required Polygonatum cyrtonema

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin Polygonatum cyrtonema
-
 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Polygonatum cyrtonema
-
 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Polygonatum cyrtonema
-
 prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.1 Polygonatum cyrtonema
-
-
-
1.17.7.4 Polygonatum cyrtonema
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.7.4 rhizome
-
 Polygonatum cyrtonema
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
-
 Polygonatum cyrtonema (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Polygonatum cyrtonema 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Polygonatum cyrtonema prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.7.1 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase
-
 Polygonatum cyrtonema
1.17.7.1 IspG
-
 Polygonatum cyrtonema
1.17.7.4 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
-
 Polygonatum cyrtonema
1.17.7.4 ispH
-
 Polygonatum cyrtonema

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.7.1 Ferredoxin
-
 Polygonatum cyrtonema
1.17.7.4 Ferredoxin
-
 Polygonatum cyrtonema

General Information

EC Number General Information Comment Organism
1.17.7.1 metabolism the content of total polysaccharide and total saponin is positively correlated with the expression patterns of several enzymes including the 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF, EC 4.6.1.12), (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ispG), and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH, EC 1.17.7.4), transcriptome analysis and determination of a link between polysaccharides and saponin biosynthesis and gene expression, overview. For saponin biosynthesis pathway, the genes encoding HMGS, MVK, ispF, ispG, ispH and FPPS enzymes are high expressed in three-year rhizomes, and these gene expression pattern is consistent with the accumulation pattern of total saponin with the rhizome development. It seems that MEP and MVA pathway all participate in the saponin biosynthesis Polygonatum cyrtonema
1.17.7.4 metabolism the content of total polysaccharide and total saponin is positively correlated with the expression patterns of several enzymes including 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF, EC 4.6.1.12), (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ispG, EC 1.17.7.1), and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH), transcriptome analysis and determination of a link between polysaccharides and saponin biosynthesis and gene expression, detailed overview. For saponin biosynthesis pathway, the genes encoding HMGS, MVK, ispF, ispG, ispH and FPPS enzymes are high expressed in three-year rhizomes, and these gene expression pattern is consistent with the accumulation pattern of total saponin with the rhizome development. It seems that MEP and MVA pathway all participate in the saponin biosynthesis Polygonatum cyrtonema