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Literature summary extracted from

  • Umezawa, S.; Akao, H.; Kubota, M.; Kino, K.
    Chemoenzymatic oxygenation method for sesquiterpenoid synthesis based on Fe-chelate and ferric-chelate reductase (2020), Biosci. Biotechnol. Biochem., 84, 780-788.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.1.9 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.9 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.1.9 HisTrap column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16.1.9 0.0035
-
 with Fe(III)-cyclam as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0204
-
 with Fe(III)-diethylenetriaminepentaacetate as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0294
-
 with Fe(III)-(citrate)2 as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0333
-
 with Fe(III)-tris(2-pyridylmethyl)amine as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0393
-
 with Fe(III)-EDTA as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0408
-
 with Fe(III)-(1,10-phenanthroline)3 as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0454
-
 with Fe(III)-(catechol)2 as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.047
-
 with Fe(III)-nitrilotriacetic acid as substrate, at pH 7.0 and 30°C Escherichia coli
1.16.1.9 0.0907
-
 with Fe(III)-(L-DOPA)2 as substrate, at pH 7.0 and 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.9 2 Fe(III)-(1,10-phenanthroline)3 + NADPH
-
 Escherichia coli 2 Fe(II)-(1,10-phenanthroline)3 + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-(catechol)2 + NADPH
-
 Escherichia coli 2 Fe(II)-(catechol)2 + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-(citrate)2 + NADPH
-
 Escherichia coli 2 Fe(II)-(citrate)2 + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-(L-DOPA)2 + NADPH highest specific activity Escherichia coli 2 Fe(II)-(L-DOPA)2 + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-cyclam + NADPH lowest specific activity Escherichia coli 2 Fe(II)-cyclam + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-diethylenetriaminepentaacetate + NADPH
-
 Escherichia coli 2 Fe(II)-diethylenetriaminepentaacetate + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-EDTA + NADPH
-
 Escherichia coli 2 Fe(II)-EDTA + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-nitrilotriacetic acid + NADPH
-
 Escherichia coli 2 Fe(II)-nitrilotriacetic acid + NADP+ + H+
-
 ?
1.16.1.9 2 Fe(III)-tris(2-pyridylmethyl)amine + NADPH
-
 Escherichia coli 2 Fe(II)-tris(2-pyridylmethyl)amine + NADP+ + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.16.1.9 YqjH
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.16.1.9 NADPH
-
 Escherichia coli