EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.2 | expression in Escherichia coli BL21 (DE3) | Halalkalibacterium ligniniphilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.2 | 0.193 | - |
3-ethylcatechol | pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum | |
1.13.11.2 | 0.418 | - |
3-methylcatechol | pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.2 | Fe2+ | at 1 mM, Fe2+ leads to 22fold activity increase | Halalkalibacterium ligniniphilum | |
1.13.11.2 | Mn2+ | at 1 mM, Mn2+ leads to 230fold activity increase | Halalkalibacterium ligniniphilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 31825 | - |
calculated from sequence, wild-type enzyme | Halalkalibacterium ligniniphilum |
1.13.11.2 | 32000 | - |
gel filtration | Halalkalibacterium ligniniphilum |
1.13.11.2 | 33988 | - |
calculated from sequence, recombinant His-tagged enzyme | Halalkalibacterium ligniniphilum |
1.13.11.2 | 34000 | - |
SDS-PAGE, recombinant His-tagged enzyme | Halalkalibacterium ligniniphilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.2 | Halalkalibacterium ligniniphilum | WP_017726464.1 | - |
- |
1.13.11.2 | Halalkalibacterium ligniniphilum L1 | WP_017726464.1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.2 | - |
Halalkalibacterium ligniniphilum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 0.0082 | - |
substrate: catechol, pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.2 | 2,3-dihydroxybiphenylcatechol + O2 | % of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum | ? | - |
? | |
1.13.11.2 | 3-ethylcatechol + O2 | best substrate. The enzyme can cleave the catechol ring when substitutions occur at C3 more efficiently than C4 substitutes. The enzyme can cleave aromatic compounds with two 6-carbon ring structures | Halalkalibacterium ligniniphilum | ? | - |
? | |
1.13.11.2 | 3-isopropylcatechol + O2 | 50% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum | ? | - |
? | |
1.13.11.2 | 3-isopropylcatechol + O2 | 50% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum L1 | ? | - |
? | |
1.13.11.2 | 3-methylcatechol + O2 | 62.5% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
1.13.11.2 | 3-methylcatechol + O2 | 62.5% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum L1 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
1.13.11.2 | 4-methylcatechol + O2 | 2.7% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum | 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.2 | 4-methylcatechol + O2 | 2.7% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum L1 | 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate | - |
? | |
1.13.11.2 | catechol + O2 | 1.5% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum | 2-hydroxymuconate-6-semialdehyde | - |
? | |
1.13.11.2 | catechol + O2 | 1.5% of the activity as compared to 3-ethylcatechol | Halalkalibacterium ligniniphilum L1 | 2-hydroxymuconate-6-semialdehyde | - |
? | |
1.13.11.2 | additional information | no activities observed for pyrogallol and 3-fluorocatechol | Halalkalibacterium ligniniphilum | ? | - |
- |
|
1.13.11.2 | additional information | no activities observed for pyrogallol and 3-fluorocatechol | Halalkalibacterium ligniniphilum L1 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.2 | monomer | under native conditions | Halalkalibacterium ligniniphilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.2 | C23Os | - |
Halalkalibacterium ligniniphilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 32.5 | - |
- |
Halalkalibacterium ligniniphilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 25 | 45 | 25°C: about 90% of maximal activity, 45°C: about 60% of maximal activity | Halalkalibacterium ligniniphilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 32.5 | 35 | 3 h, no loss of activity | Halalkalibacterium ligniniphilum |
1.13.11.2 | 40 | - |
half-life: 231 min | Halalkalibacterium ligniniphilum |
1.13.11.2 | 52 | - |
half-life: 116 min | Halalkalibacterium ligniniphilum |
1.13.11.2 | 54 | - |
half-life: 32 min | Halalkalibacterium ligniniphilum |
1.13.11.2 | 56 | - |
half-life: 4 min | Halalkalibacterium ligniniphilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.2 | 0.2 | - |
3-methylcatechol | pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum | |
1.13.11.2 | 0.47 | - |
3-ethylcatechol | pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.2 | 7.4 | - |
- |
Halalkalibacterium ligniniphilum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.13.11.2 | Halalkalibacterium ligniniphilum | calculated from sequence, recombinant His-tagged enzyme | - |
5.39 |
1.13.11.2 | Halalkalibacterium ligniniphilum | calculated from sequence, wild-type enzyme | - |
5.5 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.2 | 0.48 | - |
3-methylcatechol | pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum | |
1.13.11.2 | 2.4 | - |
3-ethylcatechol | pH 7.4, 32.5°C | Halalkalibacterium ligniniphilum |