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Literature summary extracted from
- Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer (2021), Sci. Rep., 11, 14994 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.14.3 | analysis | fusion of circularly permuted Venus, a bright variant of yellow fluorescent protein, to the C-terminus of subunit LuxB to induce bioluminescence resonance energy transfer (BRET). By using decanal as added substrate, color change and ten-times enhancement of brightness is achieved in Escherichia coli upon expression. Expression of the Venus-fused luciferase in human embryonic kidney cell lines or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhances the autonomous bioluminescence | Photorhabdus luminescens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.3 | - |
Photorhabdus luminescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.3 | Photorhabdus luminescens | P23146 and P19840 | P23146 i.e. subunit LuxA, P19840 i.e. subunit LuxB | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.3 | decanal + FMNH2 + O2 | - |
Photorhabdus luminescens | decanoic acid + FMN + H2O + hv | - |
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Release 2023.1 (January 2023)
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