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Literature summary extracted from

  • Li, J.; Li, Z.; Cao, M.; Liu, J.
    Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3 (2021), Protein Expr. Purif., 188, 105964 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.13.11.2 environmental protection the enzyme also showed resistance to most of the metal ions, surfactants and organic solvents, being a promising biocatalyst for biodegradation of aromatic compounds in complex environments Oceanimonas marisflavi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli BL21 (DE3) Oceanimonas marisflavi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0112
-
 catechol pH and temperature not specified in the publication Oceanimonas marisflavi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 69200
-
-
 Oceanimonas marisflavi

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Oceanimonas marisflavi
-
-
-
1.13.11.2 Oceanimonas marisflavi 102-Na3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2 purified 35.3fold with a yield of 46.1% Oceanimonas marisflavi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-chlorocatechol + O2
-
 Oceanimonas marisflavi 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-chlorocatechol + O2
-
 Oceanimonas marisflavi 102-Na3 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Oceanimonas marisflavi 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Oceanimonas marisflavi 102-Na3 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-chlorocatechol + O2
-
 Oceanimonas marisflavi 5-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Oceanimonas marisflavi 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Oceanimonas marisflavi 102-Na3 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Oceanimonas marisflavi 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Oceanimonas marisflavi 102-Na3 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 pyrogallol + O2
-
 Oceanimonas marisflavi ?
-
 ?
1.13.11.2 pyrogallol + O2
-
 Oceanimonas marisflavi 102-Na3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 dimer
-
 Oceanimonas marisflavi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 30
-
-
 Oceanimonas marisflavi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 45
-
 highly stable below Oceanimonas marisflavi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 8
-
-
 Oceanimonas marisflavi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.2 6 9.5 highly stable in the pH-range 6.0-9.5 Oceanimonas marisflavi