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Literature summary extracted from

  • Bocanegra-Jimenez, F.Y.; Montero-Moran, G.M.; Lara-Gonzalez, S.
    Purification and characterization of an FeII- and alpha-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae (2021), Protein Expr. Purif., 183, 105862 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.48 expression in Escherichia coli Aspergillus oryzae

General Stability

EC Number General Stability Organism
1.14.11.48 protein is stabilized in solution in the presence of either 600 microM xanthine, 1 M NaCl, 600 microM 2-oxoglutarate or 20% glycerol Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.48 NaCl decrease of up to 46% in the relative activity in presence of 0.8 M NaCl Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.48 40400
-
 dynamic light scattering Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.48 Aspergillus oryzae Q2UP14
-
-
1.14.11.48 Aspergillus oryzae ATCC 42149 Q2UP14
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.48 recombinant protein, the nickel affinity purified protein is subjected to ion-exchange chromatography once the N-terminal 6XHis-tag has been successfully removed, followed by size-exclusion purification Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.48 xanthine + 2-oxoglutarate + O2
-
 Aspergillus oryzae urate + succinate + CO2
-
 ?
1.14.11.48 xanthine + 2-oxoglutarate + O2
-
 Aspergillus oryzae ATCC 42149 urate + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.48 monomer 1 * 41720, calculated from sequence, 1 * 40000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.14.11.48 AO090001000152
-
 Aspergillus oryzae
1.14.11.48 TauD domain-containing protein
-
 Aspergillus oryzae
1.14.11.48 XanA
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.48 25
-
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.11.48 additional information
-
 protein is stabilized in solution in the presence of either 600 microM xanthine, 1 M NaCl, 600 microM 2-oxoglutarate or 20% glycerol, which increases the melting temperature by 2, 4, 5 and 6 K, respectively Aspergillus oryzae
1.14.11.48 4 25 stable within Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.48 6.5
-
-
 Aspergillus oryzae