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Literature summary extracted from

  • Li, J.; Shen, M.; Chen, Z.; Pan, F.; Yang, Y.; Shu, M.; Chen, G.; Jiao, Y.; Zhang, F.; Linhardt, R.; Zhong, W.
    Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q (2021), Protein Expr. Purif., 178, 105767 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.5 expression in Escherichia coli Pseudomonas sp. JY-Q

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.5 0.225
-
 Nicotine 37°C, pH not specified in the publication Pseudomonas sp. JY-Q
1.5.3.5 0.346
-
 Nicotine 37°C, pH not specified in the publication Pseudomonas sp. JY-Q

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.5 Pseudomonas sp. JY-Q
-
-
-
1.5.3.5 Pseudomonas sp. JY-Q A0A0S4IEN1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.5 recombinant protein Pseudomonas sp. JY-Q

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.5 nicotine + H2O + O2
-
 Pseudomonas sp. JY-Q N-methylmyosmine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.5 ? x * 55700, SDS-PAGE Pseudomonas sp. JY-Q
1.5.3.5 ? x * 56100, SDS-PAGE Pseudomonas sp. JY-Q

Synonyms

EC Number Synonyms Comment Organism
1.5.3.5 NicA2
-
 Pseudomonas sp. JY-Q
1.5.3.5 NOX
-
 Pseudomonas sp. JY-Q

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.5 35
-
-
 Pseudomonas sp. JY-Q
1.5.3.5 40
-
-
 Pseudomonas sp. JY-Q

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.5 60
-
 38% residual activity after 120 min Pseudomonas sp. JY-Q
1.5.3.5 60
-
 50% residual activity after 35 min Pseudomonas sp. JY-Q

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.5 7 7.5
-
 Pseudomonas sp. JY-Q
1.5.3.5 7.5
-
-
 Pseudomonas sp. JY-Q

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.5 FAD
-
 Pseudomonas sp. JY-Q
1.5.3.5 NAD+
-
 Pseudomonas sp. JY-Q

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.5 0.018
-
 Nicotine 37°C, pH not specified in the publication Pseudomonas sp. JY-Q
1.5.3.5 0.024
-
 Nicotine 37°C, pH not specified in the publication Pseudomonas sp. JY-Q