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Literature summary extracted from

  • Shen, W.; Han, J.; Yan, P.; Zheng, J.; Zhang, L.; Li, X.; Tuo, D.; Zhou, P.
    Soluble expression of biologically active methionine sulfoxide reductase B1 (PaMsrB1) from Carica papaya in Escherichia coli and isolation of its protein targets (2018), Protein Expr. Purif., 146, 17-22 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 PaMsrB1 as a fusion protein containing an N-terminal maltose-binding protein (MBP) is expressed in Escherichia coli Rosetta (DE3) cells. Production of soluble fusion protein is greater when the cells are cultured at 16 °C than at 37°C Carica papaya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.12 16900
-
 SDS-PAGE Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Carica papaya F2YS22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12
-
 Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 dabsyl-L-methionine (R)-sulfoxide + dithiothreitol
-
 Carica papaya dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
 ?
1.8.4.12 L-methionine-(R)-S-oxide + dithiothreitol
-
 Carica papaya L-methionine + dithiothreitol disulfide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.12 ? x * 16900, SDS-PAGE Carica papaya

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 methionine sulfoxide reductase B1
-
 Carica papaya
1.8.4.12 PaMsrB1
-
 Carica papaya

General Information

EC Number General Information Comment Organism
1.8.4.12 physiological function methionine sulfoxide reductase B1 (MsrB1) protects the photosynthetic apparatus from oxidative damage by scavenging reactive oxygen species to repair Met-oxidized proteins in response to abiotic stresses and biotic attack Carica papaya