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Literature summary extracted from
- Purification and characterization of a novel manganese peroxidase from white-rot fungus Cerrena unicolor BBP6 and its application in dye decolorization and denim bleaching (2018), Process Biochem., 66, 222-229 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | ferulic acid | - |
Cerrena unicolor |  |
| 1.11.1.13 | gallic acid | - |
Cerrena unicolor | |
| 1.11.1.13 | Tween 80 | - |
Cerrena unicolor | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.11.1.13 | Ca2+ reduces the enzyme stability by 40% at 10 mM | Cerrena unicolor |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | Ca2+ | 30% inhibition at 10 mM, Ca2+ reduces the enzyme stability by 40% | Cerrena unicolor | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.13 | 0.0321 | - |
H2O2 | pH 4.8, 30°C | Cerrena unicolor | |
| 1.11.1.13 | 0.0467 | - |
Mn(II) | pH 4.8, 30°C | Cerrena unicolor | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.11.1.13 | extracellular | manganese peroxidase (MnP) is an extracellular glycosylated heme protein | Cerrena unicolor | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | Ca2+ | required, enzyme-bound, 40% inhibition at 10 mM | Cerrena unicolor | |
| 1.11.1.13 | Fe2+ | in the heme group | Cerrena unicolor | |
| 1.11.1.13 | Mg2+ | slightly activating at 10 mM | Cerrena unicolor | |
| 1.11.1.13 | Mn2+ | 20% activating at 10 mM | Cerrena unicolor | |
| 1.11.1.13 | additional information | poor effects by 10 mM of Zn2+, Na+, and K+ | Cerrena unicolor |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 | Cerrena unicolor | - |
2 Mn(III) + 2 H2O | - |
? | |
| 1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 | Cerrena unicolor BBP6 | - |
2 Mn(III) + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.13 | Cerrena unicolor | A0A7D5FUQ6 | - |
- |
| 1.11.1.13 | Cerrena unicolor BBP6 | A0A7D5FUQ6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.13 | native enzyme 6.9fold by anion exchange chromatography | Cerrena unicolor |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 112.4 | - |
purified enzyme, pH 4.8, 30°C | Cerrena unicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 | - |
Cerrena unicolor | 2 Mn(III) + 2 H2O | - |
? | |
| 1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 | - |
Cerrena unicolor BBP6 | 2 Mn(III) + 2 H2O | - |
? | |
| 1.11.1.13 | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H+ + H2O2 | - |
Cerrena unicolor | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O | - |
? | |
| 1.11.1.13 | 2,6-dimethoxyphenol + 2 H+ + H2O2 | - |
Cerrena unicolor | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
| 1.11.1.13 | brilliant blue R + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
| 1.11.1.13 | bromophenol blue + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
| 1.11.1.13 | bromophenol blue + H2O2 | dye decolorization | Cerrena unicolor BBP6 | ? | - |
? | |
| 1.11.1.13 | Congo red + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
| 1.11.1.13 | crystal violet + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
| 1.11.1.13 | crystal violet + H2O2 | dye decolorization | Cerrena unicolor BBP6 | ? | - |
? | |
| 1.11.1.13 | guaiacol + 2 H+ + H2O2 | - |
Cerrena unicolor | oxidized guaiacol + 2 H2O | - |
? | |
| 1.11.1.13 | methyl orange + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
| 1.11.1.13 | additional information | evaluation of the dye decolorization ability of the purified enzyme, MnP-BBP6, the enzyme is used to decolorize different types of synthetic dyes including RBBR, Congo red (CR), brilliant blue R (BBR), methyl orange (MO), bromophenol blue (BPB), and crystal violet (CV). Denim bleaching by the purified MnP-BBP6 | Cerrena unicolor | ? | - |
- |
|
| 1.11.1.13 | additional information | evaluation of the dye decolorization ability of the purified enzyme, MnP-BBP6, the enzyme is used to decolorize different types of synthetic dyes including RBBR, Congo red (CR), brilliant blue R (BBR), methyl orange (MO), bromophenol blue (BPB), and crystal violet (CV). Denim bleaching by the purified MnP-BBP6 | Cerrena unicolor BBP6 | ? | - |
- |
|
| 1.11.1.13 | Remazol Brilliant Blue R + H2O2 | dye decolorization | Cerrena unicolor | ? | - |
? | |
| 1.11.1.13 | Remazol Brilliant Blue R + H2O2 | dye decolorization | Cerrena unicolor BBP6 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | ? | x * 45000, SDS-PAGE | Cerrena unicolor |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | MnP | - |
Cerrena unicolor |
| 1.11.1.13 | MnP-BBP6 | - |
Cerrena unicolor |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 60 | - |
- |
Cerrena unicolor |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 20 | - |
purified enzyme, pH 4.8, 90% activity remaining | Cerrena unicolor |
| 1.11.1.13 | 40 | - |
purified enzyme, pH 4.8, completely stable at | Cerrena unicolor |
| 1.11.1.13 | 60 | - |
purified enzyme, pH 4.8, 75% activity remaining | Cerrena unicolor |
| 1.11.1.13 | 80 | - |
purified enzyme, pH 4.8, inactivation | Cerrena unicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 4.8 | - |
- |
Cerrena unicolor |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 3.5 | 5.5 | over 50% of maximal activity within this range | Cerrena unicolor |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 3 | 7.5 | purified enzyme, 30°C, full stability at pH 4.5, below 10% activity remaining at pH 3.0 and pH 7.5, 90% at pH 4.0, 80% at pH 5.0, and 30% at pH 6.0 | Cerrena unicolor |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | heme | - |
Cerrena unicolor | |
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