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Literature summary extracted from

  • Uchendu, S.; Rafalowski, A.; Schick, D.; Cohn, E.; Hassan, B.; Taylor, E.
    Identifying metabolic pathway intermediates that modulate the gallate dioxygenase (DesB) from Sphingobium sp. strain SYK-6 (2021), Process Biochem., 102, 408-416 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.57 expressed in Escherichia coli BL21(DE3) cells Sphingobium sp. SYK-6

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.57 2-Methoxy-4-methylphenol 20.8% inhibition at 5 mM Sphingobium sp. SYK-6
1.13.11.57 4-nitrocatechol 31.6% inhibition at 5 mM Sphingobium sp. SYK-6
1.13.11.57 protocatechuate most potent inhibitor with mixed (competitive and non-competitive) inhibition profile, 49% inhibition at 5 mM Sphingobium sp. SYK-6
1.13.11.57 syringaldehyde least potent inhibitor, 8.66% inhibition at 5 mM Sphingobium sp. SYK-6
1.13.11.57 syringate 16.3% inhibition at 5 mM Sphingobium sp. SYK-6
1.13.11.57 vanilliate 24.2% inhibition at 5 mM Sphingobium sp. SYK-6
1.13.11.57 Vanillin 11.3% inhibition at 5 mM Sphingobium sp. SYK-6

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.57 0.123
-
 gallate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.57 Sphingobium sp. SYK-6 G2IKE5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.57 Sephadex G-25 gel filtration Sphingobium sp. SYK-6

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.57 gallate + O2
-
 Sphingobium sp. SYK-6 (2E,4E)-4-(carboxymethylidene)-2-hydroxypent-2-enedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.57 ? x * 49220, calculated from amino acid sequence Sphingobium sp. SYK-6

Synonyms

EC Number Synonyms Comment Organism
1.13.11.57 DesB
-
 Sphingobium sp. SYK-6
1.13.11.57 gallate 3,4-dioxygenase
-
 Sphingobium sp. SYK-6

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.57 316
-
 gallate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.57 5.48
-
 protocatechuate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6
1.13.11.57 8.13
-
 vanilliate at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6
1.13.11.57 13.3
-
 4-nitrocatechol at pH 7.5, temperature not specified in the publication Sphingobium sp. SYK-6