Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hiraga, Y.; Shimada, N.; Nagashima, Y.; Suda, K.; Kanamori, T.; Ishiguro, K.; Sato, Y.; Hirakawa, H.; Sato, S.; Akashi, T.; Tanaka, Y.; Ohta, D.; Aoki, K.; Shibata, D.; Suzuki, H.; Kera, K.
    Identification of a flavin monooxygenase-like flavonoid 8-hydroxylase with gossypetin synthase activity from Lotus japonicus (2021), Plant Cell Physiol., 62, 411-423 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.19 gene LjB13E21 or LjF8H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant enzyme expression in Arabidopsis thaliana strain Col-0 seedlings under the control of a CaMV35S promoter using a pBE2113 vector via transfection method using Agrobacterium tumefaciens strain (GV3101::pMP90), and recombinant expression in Saccharomyces cerevisiae strain BJ2168 microsomes, semi-quantitative RT-PCR enzyme expression analysis Lotus japonicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.19 microsome
-
 Lotus japonicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.19 taxifolin + NADPH + H+ + O2 Lotus japonicus
-
 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?
1.14.13.19 taxifolin + NADPH + H+ + O2 Lotus japonicus Miyakojima MG-20
-
 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.19 Lotus japonicus A0A830U7D1 Lotus corniculatus var. japonicus
-
1.14.13.19 Lotus japonicus Miyakojima MG-20 A0A830U7D1 Lotus corniculatus var. japonicus
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.19 flower LjF8H has high relative expression in the early flowering developmental stage Lotus japonicus
-
1.14.13.19 flower bud
-
 Lotus japonicus
-
1.14.13.19 additional information LjF8H expression pattern, overview Lotus japonicus
-
1.14.13.19 petal
-
 Lotus japonicus
-
1.14.13.19 seedling
-
 Lotus japonicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.19 apigenin + NADPH + H+ + O2
-
 Lotus japonicus isoscutellarein + NADP+ + H2O
-
 ?
1.14.13.19 apigenin + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 isoscutellarein + NADP+ + H2O
-
 ?
1.14.13.19 eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus 6-hydroxyeriodictyol + NADP+ + H2O
-
 ?
1.14.13.19 eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 6-hydroxyeriodictyol + NADP+ + H2O
-
 ?
1.14.13.19 eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus 8-hydroxyeriodictyol + NADP+ + H2O
-
 ?
1.14.13.19 eriodictyol + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 8-hydroxyeriodictyol + NADP+ + H2O
-
 ?
1.14.13.19 kaempferol + NADPH + H+ + O2
-
 Lotus japonicus herbacetin + NADP+ + H2O
-
 ?
1.14.13.19 luteolin + NADPH + H+ + O2
-
 Lotus japonicus 8-hydroxyluteolin + NADP+ + H2O
-
 ?
1.14.13.19 additional information broad substrate acceptance of enzyme LjF8H, overview Lotus japonicus ?
-
-
1.14.13.19 additional information broad substrate acceptance of enzyme LjF8H, overview Lotus japonicus Miyakojima MG-20 ?
-
-
1.14.13.19 naringenin + NADPH + H+ + O2
-
 Lotus japonicus isocarthamidin + NADP+ + H2O
-
 ?
1.14.13.19 quercetin + NADPH + H+ + O2
-
 Lotus japonicus gossypetin + NADP+ + H2O
-
 ?
1.14.13.19 taxifolin + NADPH + H+ + O2
-
 Lotus japonicus 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?
1.14.13.19 taxifolin + NADPH + H+ + O2
-
 Lotus japonicus Miyakojima MG-20 2,3-dihydrogossypetin + NADP+ + H2O
-
 ?
1.14.13.19 taxifolin + NADPH + H+ + O2
-
 Lotus japonicus 6-hydroxytaxifolin + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.19 F8H
-
 Lotus japonicus
1.14.13.19 flavin monooxygenase-like flavonoid 8-hydroxylase
-
 Lotus japonicus
1.14.13.19 flavonoid 8-hydroxylase
-
 Lotus japonicus
1.14.13.19 LjF8H
-
 Lotus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.19 30
-
 assay at Lotus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.19 7
-
 assay at Lotus japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.19 FAD
-
 Lotus japonicus
1.14.13.19 NADPH
-
 Lotus japonicus

General Information

EC Number General Information Comment Organism
1.14.13.19 evolution Lotus japonicus synthesizes and accumulates gossypetin glycosides in flower, thereby impacting on the tone of the yellow color in petals, possible F8H responsible for gossypetin synthesis in petals. Enzyme LjF8H is class-A flavoprotein monooxygenase Lotus japonicus
1.14.13.19 physiological function enzyme LjF8H has high relative expression in the early flowering developmental stage, implying that LjF8H may also contribute for flower pigment synthesis before flowering Lotus japonicus