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Literature summary extracted from

  • Hawkes, T.R.; Langford, M.P.; Viner, R.; Blain, R.E.; Callaghan, F.M.; Mackay, E.A.; Hogg, B.V.; Singh, S.; Dale, R.P.
    Characterization of 4-hydroxyphenylpyruvate dioxygenases, inhibition by herbicides and engineering for herbicide tolerance in crops (2019), Pestic. Biochem. Physiol., 156, 9-28.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.27 A327R/L359M about 90% of wild-type activity Avena sativa
1.13.11.27 G411A about 15% of wild-type activity Avena sativa
1.13.11.27 G413W about 20% of wild-type activity Avena sativa
1.13.11.27 L359M about 80% of wild-type activity Avena sativa
1.13.11.27 P315R/R328A/L347M mutations induce an 8fold increase in the value of k4 in respect of mesotrione Arabidopsis thaliana
1.13.11.27 V218I/A327R/I340E/L359M about 165% of wild-type activity Avena sativa
1.13.11.27 V218I/A327R/I340E/L359M/G411A about 6% of wild-type activity Avena sativa
1.13.11.27 V218I/A327R/L359M about 80% of wild-type activity Avena sativa
1.13.11.27 V218L/A327R/I340E/L359M about 170% of wild-type activity Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 0.0017
-
 4-hydroxyphenylpyruvate mutant V218L/A327R/I340E/L359M , pH 7, 25°C Avena sativa
1.13.11.27 0.002
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M, pH 7, 25°C Avena sativa
1.13.11.27 0.0026
-
 4-hydroxyphenylpyruvate mutant A327R/L359M, pH 7, 25°C Avena sativa
1.13.11.27 0.00304
-
 4-hydroxyphenylpyruvate pH 7, 25°C Arabidopsis thaliana
1.13.11.27 0.0031
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/L359M, pH 7, 25°C Avena sativa
1.13.11.27 0.0043
-
 4-hydroxyphenylpyruvate mutant L359M, pH 7, 25°C Avena sativa
1.13.11.27 0.0048
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Avena sativa
1.13.11.27 0.0143
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C Avena sativa
1.13.11.27 0.0309
-
 4-hydroxyphenylpyruvate mutant G413W, pH 7, 25°C Avena sativa
1.13.11.27 0.0389
-
 4-hydroxyphenylpyruvate mutant G411A, pH 7, 25°C Avena sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.27 Iron 0.37 atoms per protomer Arabidopsis thaliana
1.13.11.27 Iron 0.37 atoms per protomer Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Arabidopsis thaliana P93836
-
-
1.13.11.27 Avena sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Arabidopsis thaliana homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Avena sativa homogentisate + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.27 1.25
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C Avena sativa
1.13.11.27 3.52
-
 4-hydroxyphenylpyruvate mutant A327R/L359M, pH 7, 25°C Avena sativa
1.13.11.27 4.25
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Arabidopsis thaliana
1.13.11.27 4.54
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/L359M, pH 7, 25°C Avena sativa
1.13.11.27 4.54
-
 4-hydroxyphenylpyruvate mutant V218L/A327R/I340E/L359M , pH 7, 25°C Avena sativa
1.13.11.27 4.96
-
 4-hydroxyphenylpyruvate mutant G411A, pH 7, 25°C Avena sativa
1.13.11.27 4.99
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M, pH 7, 25°C Avena sativa
1.13.11.27 5.04
-
 4-hydroxyphenylpyruvate mutant L359M, pH 7, 25°C Avena sativa
1.13.11.27 6.97
-
 4-hydroxyphenylpyruvate mutant G413W, pH 7, 25°C Avena sativa
1.13.11.27 7.27
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Avena sativa
1.13.11.27 8.25
-
 4-hydroxyphenylpyruvate mutant P315R/R328A/L347M, pH 7, 25°C Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.27 90
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C Avena sativa
1.13.11.27 140
-
 4-hydroxyphenylpyruvate mutant G411A, pH 7, 25°C Avena sativa
1.13.11.27 230
-
 4-hydroxyphenylpyruvate mutant G413W, pH 7, 25°C Avena sativa
1.13.11.27 1210
-
 4-hydroxyphenylpyruvate mutant L359M, pH 7, 25°C Avena sativa
1.13.11.27 1370
-
 4-hydroxyphenylpyruvate mutant A327R/L359M, pH 7, 25°C Avena sativa
1.13.11.27 1410
-
 4-hydroxyphenylpyruvate pH 7, 25°C Arabidopsis thaliana
1.13.11.27 1570
-
 4-hydroxyphenylpyruvate wild-type, pH 7, 25°C Avena sativa
1.13.11.27 1610
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/L359M, pH 7, 25°C Avena sativa
1.13.11.27 2510
-
 4-hydroxyphenylpyruvate mutant V218I/A327R/I340E/L359M, pH 7, 25°C Avena sativa
1.13.11.27 2660
-
 4-hydroxyphenylpyruvate mutant V218L/A327R/I340E/L359M , pH 7, 25°C Avena sativa