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Literature summary extracted from

  • Pennington, J.; Kemp, M.; McGarry, L.; Chen, Y.; Stroupe, M.
    Siroheme synthase orients substrates for dehydrogenase and chelatase activities in a common active site (2020), Nat. Commun., 11, 864 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.76 expressed in Escherichia coli LMG194 cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.76 S128A mutant enzyme bound to precorrin-2, sirohydrochlorin, and a cobalt-sirohydrochlorin product, hanging drop vapor diffusion method, using 100 mM MES pH 5.0, 500 mM NaCl, 1% (v/v) 2-mercaptoethanol and 10-20% (w/v) PEG4000 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.76 D104A inactive Salmonella enterica subsp. enterica serovar Typhimurium
1.3.1.76 P133H inactive Salmonella enterica subsp. enterica serovar Typhimurium
1.3.1.76 R260A inactive Salmonella enterica subsp. enterica serovar Typhimurium
1.3.1.76 S128A the variant is more active for dehydrogenation and chelation than wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+ Salmonella enterica subsp. enterica serovar Typhimurium
-
 sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 sirohydrochlorin + Fe2+ Salmonella enterica subsp. enterica serovar Typhimurium
-
 siroheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.76 Salmonella enterica subsp. enterica serovar Typhimurium P25924
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.76 Ni-NTA column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.76 precorrin-2 + NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium sirohydrochlorin + NADH + H+
-
 ?
1.3.1.76 sirohydrochlorin + Fe2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium siroheme + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.76 CysG
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.3.1.76 siroheme synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.76 NAD+ dependent on Salmonella enterica subsp. enterica serovar Typhimurium