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Literature summary extracted from

  • Mayol, O.; Bastard, K.; Beloti, L.; Frese, A.; Turkenburg, J.; Petit, J.; Mariage, A.; Debard, A.; Pellouin, V.; Perret, A.; de Berardinis, V.; Zaparucha, A.; Grogan, G.; Vergne-Vaxelaire, C.
    A family of native amine dehydrogenases for the asymmetric reductive amination of ketones (2019), Nat. Catal., 2, 324-333 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.28 0.01
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.06
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.06
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.29
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.55
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.32
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.5
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 98.9
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 392
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.28 Cystobacter fuscus S9Q235
-
-
1.4.1.28 Cystobacter fuscus DSM 2262 S9Q235
-
-
1.4.1.28 Microbacterium sp. MA1 C3UMY1
-
-
1.4.1.28 Mycolicibacterium smegmatis A0A8B4R7U8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.28 2.3
-
 substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.3
-
 substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.7
-
 substrate 3-buten-2-one, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 3 8 substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 3.6
-
 substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 4.3
-
 substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 5.6
-
 substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 7.4
-
 substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 7.9
-
 substrate 3-buten-2-one, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 9.3
-
 substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 14.9
-
 substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 18.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 18.8
-
 substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 19.6
-
 substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 25.1
-
 substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 25.5
-
 substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 33.8
-
 substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 34.2
-
 substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 35
-
 substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 37.1
-
 substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 37.3
-
 substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 40.1
-
 substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 41.5
-
 substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 54.4
-
 substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 60.1
-
 substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 60.2
-
 substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 60.6
-
 substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 63.6
-
 substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 85.2
-
 substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 109.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 120.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 129
-
 substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 160.9
-
 substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 161
-
 substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 170.1
-
 substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 175.2
-
 substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 196.2
-
 substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 206.5
-
 substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 233.2
-
 substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 245.6
-
 substrate 3-methylbutan-2-one, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 252.6
-
 substrate 2-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 337
-
 substrate 2-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 389
-
 substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 614
-
 substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C Microbacterium sp. MA1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-cyclohexen-1-amine + H2O + NAD+
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 3-buten-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-buten-2-amine + H2O + NAD+
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylbutan-2-amine + H2O + NAD+
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylbutan-2-amine + H2O + NAD+
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylbutan-2-amine + H2O + NAD+
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 3-methylbutan-2-amine + H2O + NAD+
-
 ?
1.4.1.28 3-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 3-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 3-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 4-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 4-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 4-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 4-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 4-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 4-methylcyclohexylamine + H2O + NAD+
-
 ?
1.4.1.28 cyclohexanone + methylamine + NADPH + H+
-
 Mycolicibacterium smegmatis N-methylcyclohexanamine + H2O + NADP+
-
 ?
1.4.1.28 cyclohexanone + methylamine + NADPH + H+
-
 Cystobacter fuscus N-methylcyclohexanamine + H2O + NADP+
-
 ?
1.4.1.28 cyclohexanone + methylamine + NADPH + H+
-
 Microbacterium sp. MA1 N-methylcyclohexanamine + H2O + NADP+
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis cyclopentylamine + H2O + NAD+
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus cyclopentylamine + H2O + NAD+
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 cyclopentylamine + H2O + NAD+
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 cyclopentylamine + H2O + NAD+
-
 ?
1.4.1.28 additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Mycolicibacterium smegmatis ?
-
-
1.4.1.28 additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Cystobacter fuscus ?
-
-
1.4.1.28 additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Microbacterium sp. MA1 ?
-
-
1.4.1.28 additional information also reacts with aldehydes such as 2-methylbutanal, isobutyraldehyde Cystobacter fuscus DSM 2262 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.4.1.28 AMDH
-
 Mycolicibacterium smegmatis
1.4.1.28 AMDH
-
 Cystobacter fuscus
1.4.1.28 AMDH
-
 Microbacterium sp. MA1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.28 0.04
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.05
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.1
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.28
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.3
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.58
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.61
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.94
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 13
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.28 0.0008
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.261
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.4
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 1.723
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 1.995
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 4.893
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 54.4
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 1704
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 4537
-
 NADPH pH 9.5, 23°C Cystobacter fuscus